Zainy, Zainy and Hyder, Muhammad Zeeshan and Uzma, Uzma and Fayyaz, Muhammad and Ijaz, Umer Zeeshan and Farrakh, Sumaira (2025) Comparative transcriptome analysis of resistant, moderately resistant and susceptible wheat near isogenic lines in response to Puccinia striiformis tritici. Journal of Plant Interactions, 20 (1): 2481853. ISSN 1742-9145
AI Summary:
The study used RNA seq technique to understand the wheat and stripe rust interaction in three different reaction types. The results show that although the responses of the near iso-genic lines are similar, it is the magnitude of the response that plays a role in the differences in their resistance to Puccinia striiformis.AI Topics:
Stripe/yellow rust is caused by a biotrophic fungal pathogen known as Puccinia striiformis f. sp.tritici (Pst). RNA seq technique was used to understand the wheat and stripe rust interaction in three different reaction types. Leave samples of three near-isogenic lines (NILs) of wheat showing Resistant/immune (R), Moderately Resistant (MR), and Susceptible (S) (inoculated with Puccinia striiformis race 574232) responses were collected 48 and 72 h after inoculation (hai). Overall, 8,595 DEGs were upregulated and 8,741 were downregulated with high numbers (4,357) of DEGs identified S-R in comparison at 72hai. Subsequent Gene Ontology (GO) enrichment analysis suggests protein phosphorylation, biological process, and cellular process of internal components of membrane enriched in all three comparisons at both time points. DEGs identified as serine/threonine receptor-like kinases, peroxidases, Mitogen-phosphate Kinases (MAPK), Phenylalanine ammonia-lyase (PAL), and Pathogenesis-related protein (PR) were high in resistant NIL in all comparisons at both time points. The results show that although the responses of the near iso-genic lines are similar, it is the magnitude of the response that plays a role in the differences in their resistance to Puccinia striiformis.
Zainy, Zainy
Author
Zainy, Zainy and Hyder, Muhammad Zeeshan and Uzma, Uzma and Fayyaz, Muhammad and Ijaz, Umer Zeeshan and Farrakh, Sumaira (2025) Comparative transcriptome analysis of resistant, moderately resistant and susceptible wheat near isogenic lines in response to Puccinia striiformis tritici. Journal of Plant Interactions, 20 (1): 2481853. ISSN 1742-9145
See full publications listHyder, Muhammad Zeeshan
Author
Zainy, Zainy and Hyder, Muhammad Zeeshan and Uzma, Uzma and Fayyaz, Muhammad and Ijaz, Umer Zeeshan and Farrakh, Sumaira (2025) Comparative transcriptome analysis of resistant, moderately resistant and susceptible wheat near isogenic lines in response to Puccinia striiformis tritici. Journal of Plant Interactions, 20 (1): 2481853. ISSN 1742-9145
See full publications listUzma
Author
Zainy, Zainy and Hyder, Muhammad Zeeshan and Uzma, Uzma and Fayyaz, Muhammad and Ijaz, Umer Zeeshan and Farrakh, Sumaira (2025) Comparative transcriptome analysis of resistant, moderately resistant and susceptible wheat near isogenic lines in response to Puccinia striiformis tritici. Journal of Plant Interactions, 20 (1): 2481853. ISSN 1742-9145
Bibi, Sara and Kerbiriou, Caroline and Uzma and Mckirdy, Shona and Kostrytsia, Anastasiia and Rasheed, Hifza and Eqani, Syed Ali Musstjab Akber Shah and Gerasimidis, Konstantinos and Nurulain, Syed Muhammad and Ijaz, Umer Zeeshan (2024) Gut microbiome and function are altered for individuals living in high fluoride concentration areas in Pakistan. Ecotoxicology and Environmental Safety, 284: 116959. ISSN 0147-6513
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See full publications listFayyaz, Muhammad
Author
Zainy, Zainy and Hyder, Muhammad Zeeshan and Uzma, Uzma and Fayyaz, Muhammad and Ijaz, Umer Zeeshan and Farrakh, Sumaira (2025) Comparative transcriptome analysis of resistant, moderately resistant and susceptible wheat near isogenic lines in response to Puccinia striiformis tritici. Journal of Plant Interactions, 20 (1): 2481853. ISSN 1742-9145
See full publications listIjaz, Umer
Author
Cholet, Fabien and Vignola, Marta and Quinn, Dominic and Ijaz, Umer Z. and Sloan, William T. and Smith, Cindy J. (2025) Microbial ecology of drinking water biofiltration based on 16S rRNA sequencing: a meta-analysis. Water Research, 281: 123684. ISSN 0043-1354
Saleem, Farrukh and Ameer, Aqsa and Afzal, Farhan and Usman, Muhammad and Irshad, Hamid and Sattar, Sadia and Ijaz, Umer Zeeshan and Javed, Sundus (2025) A cross-sectional survey of poultry management systems, practices and antimicrobial use in relation to disease outbreak in Pakistan. BMC Research Notes, 18: 144. ISSN 1756-0500
Badar, Ayesha and Aqueel, Rhea and Nawaz, Ali and Ijaz, Umer Zeeshan and Malik, Kauser Abdulla (2025) Microbiota transplantation for Cotton Leaf Curl Disease suppression - core microbiome and transcriptome dynamics. Communications Biology, 8 (1): 380. ISSN 2399-3642
See full publications listFarrakh, Sumaira
Author
Zainy, Zainy and Hyder, Muhammad Zeeshan and Uzma, Uzma and Fayyaz, Muhammad and Ijaz, Umer Zeeshan and Farrakh, Sumaira (2025) Comparative transcriptome analysis of resistant, moderately resistant and susceptible wheat near isogenic lines in response to Puccinia striiformis tritici. Journal of Plant Interactions, 20 (1): 2481853. ISSN 1742-9145
See full publications listAvailable under License Creative Commons Attribution.
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