Neilson, Lisa J. and Cartwright, Douglas and Risteli, Maija and Jokinen, Elina M. and McGarry, Lynn and Sandvik, Toni and Nikolatou, Konstantina and Hodge, Kelly and Atkinson, Samuel and Vias, Maria and Kay, Emily J. and Brenton, James D. and Carlin, Leo M. and Bryant, David M. and Salo, Tuula and Zanivan, Sara (2023) Omentum-derived matrix enables the study of metastatic ovarian cancer and stromal cell functions in a physiologically relevant environment. Matrix Biology Plus, 19-20: 100136. ISSN 2590-0285

Abstract

High-grade serous (HGS) ovarian cancer is the most lethal gynaecological disease in the world and metastases is a major cause. The omentum is the preferential metastatic site in HGS ovarian cancer patients and in vitro models that recapitulate the original environment of this organ at cellular and molecular level are being developed to study basic mechanisms that underpin this disease. The tumour extracellular matrix (ECM) plays active roles in HGS ovarian cancer pathology and response to therapy. However, most of the current in vitro models use matrices of animal origin and that do not recapitulate the complexity of the tumour ECM in patients.

Here, we have developed omentum gel (OmGel), a matrix made from tumour-associated omental tissue of HGS ovarian cancer patients that has unprecedented similarity to the ECM of HGS omental tumours and is simple to prepare. When used in 2D and 3D in vitro assays to assess cancer cell functions relevant to metastatic ovarian cancer, OmGel performs as well as or better than the widely use Matrigel and does not induce additional phenotypic changes to ovarian cancer cells. Surprisingly, OmGel promotes pronounced morphological changes in cancer associated fibroblasts (CAFs). These changes were associated with the upregulation of proteins that define subsets of CAFs in tumour patient samples, highlighting the importance of using clinically and physiologically relevant matrices for in vitro studies. Hence, OmGel provides a step forward to study the biology of HGS omental metastasis. Metastasis in the omentum are also typical of other cancer types, particularly gastric cancer, implying the relevance of OmGel to study the biology of other highly lethal cancers.

People
Neilson, Lisa J.
Author

Neilson, Lisa J. and Cartwright, Douglas and Risteli, Maija and Jokinen, Elina M. and McGarry, Lynn and Sandvik, Toni and Nikolatou, Konstantina and Hodge, Kelly and Atkinson, Samuel and Vias, Maria and Kay, Emily J. and Brenton, James D. and Carlin, Leo M. and Bryant, David M. and Salo, Tuula and Zanivan, Sara (2023) Omentum-derived matrix enables the study of metastatic ovarian cancer and stromal cell functions in a physiologically relevant environment. Matrix Biology Plus, 19-20: 100136. ISSN 2590-0285

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Cartwright, D.
Author

Cartwright, Douglas and Kidd, Andrew C. and Ansel, Sonam and Ascierto, Maria Libera and Spiliopoulou, Pavlina (2025) Oncogenic signalling pathways in cancer immunotherapy: leader or follower in this delicate dance? International Journal of Molecular Sciences, 26 (9): 4393. ISSN 1661-6596

Peters, A.L. and Hall, P.S. and Jordan, L.B. and Soh, F.Y. and Hannington, L. and Makaranka, S. and Urquhart, G. and Vallet, M. and Cartwright, D. and Marashi, H. and Elsberger, B. (2024) Enhancing clinical decision support with genomic tools in breast cancer: a Scottish perspective. Breast, 75: 103728. ISSN 0960-9776

Neilson, Lisa J. and Cartwright, Douglas and Risteli, Maija and Jokinen, Elina M. and McGarry, Lynn and Sandvik, Toni and Nikolatou, Konstantina and Hodge, Kelly and Atkinson, Samuel and Vias, Maria and Kay, Emily J. and Brenton, James D. and Carlin, Leo M. and Bryant, David M. and Salo, Tuula and Zanivan, Sara (2023) Omentum-derived matrix enables the study of metastatic ovarian cancer and stromal cell functions in a physiologically relevant environment. Matrix Biology Plus, 19-20: 100136. ISSN 2590-0285

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Risteli, Maija
Author

Neilson, Lisa J. and Cartwright, Douglas and Risteli, Maija and Jokinen, Elina M. and McGarry, Lynn and Sandvik, Toni and Nikolatou, Konstantina and Hodge, Kelly and Atkinson, Samuel and Vias, Maria and Kay, Emily J. and Brenton, James D. and Carlin, Leo M. and Bryant, David M. and Salo, Tuula and Zanivan, Sara (2023) Omentum-derived matrix enables the study of metastatic ovarian cancer and stromal cell functions in a physiologically relevant environment. Matrix Biology Plus, 19-20: 100136. ISSN 2590-0285

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Jokinen, Elina M.
Author

Neilson, Lisa J. and Cartwright, Douglas and Risteli, Maija and Jokinen, Elina M. and McGarry, Lynn and Sandvik, Toni and Nikolatou, Konstantina and Hodge, Kelly and Atkinson, Samuel and Vias, Maria and Kay, Emily J. and Brenton, James D. and Carlin, Leo M. and Bryant, David M. and Salo, Tuula and Zanivan, Sara (2023) Omentum-derived matrix enables the study of metastatic ovarian cancer and stromal cell functions in a physiologically relevant environment. Matrix Biology Plus, 19-20: 100136. ISSN 2590-0285

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McGarry, Lynn
Author

Neilson, Lisa J. and Cartwright, Douglas and Risteli, Maija and Jokinen, Elina M. and McGarry, Lynn and Sandvik, Toni and Nikolatou, Konstantina and Hodge, Kelly and Atkinson, Samuel and Vias, Maria and Kay, Emily J. and Brenton, James D. and Carlin, Leo M. and Bryant, David M. and Salo, Tuula and Zanivan, Sara (2023) Omentum-derived matrix enables the study of metastatic ovarian cancer and stromal cell functions in a physiologically relevant environment. Matrix Biology Plus, 19-20: 100136. ISSN 2590-0285

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Sandvik, Toni
Author

Neilson, Lisa J. and Cartwright, Douglas and Risteli, Maija and Jokinen, Elina M. and McGarry, Lynn and Sandvik, Toni and Nikolatou, Konstantina and Hodge, Kelly and Atkinson, Samuel and Vias, Maria and Kay, Emily J. and Brenton, James D. and Carlin, Leo M. and Bryant, David M. and Salo, Tuula and Zanivan, Sara (2023) Omentum-derived matrix enables the study of metastatic ovarian cancer and stromal cell functions in a physiologically relevant environment. Matrix Biology Plus, 19-20: 100136. ISSN 2590-0285

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Nikolatou, Konstantina
Author

Neilson, Lisa J. and Cartwright, Douglas and Risteli, Maija and Jokinen, Elina M. and McGarry, Lynn and Sandvik, Toni and Nikolatou, Konstantina and Hodge, Kelly and Atkinson, Samuel and Vias, Maria and Kay, Emily J. and Brenton, James D. and Carlin, Leo M. and Bryant, David M. and Salo, Tuula and Zanivan, Sara (2023) Omentum-derived matrix enables the study of metastatic ovarian cancer and stromal cell functions in a physiologically relevant environment. Matrix Biology Plus, 19-20: 100136. ISSN 2590-0285

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Hodge, Kelly
Author

Neilson, Lisa J. and Cartwright, Douglas and Risteli, Maija and Jokinen, Elina M. and McGarry, Lynn and Sandvik, Toni and Nikolatou, Konstantina and Hodge, Kelly and Atkinson, Samuel and Vias, Maria and Kay, Emily J. and Brenton, James D. and Carlin, Leo M. and Bryant, David M. and Salo, Tuula and Zanivan, Sara (2023) Omentum-derived matrix enables the study of metastatic ovarian cancer and stromal cell functions in a physiologically relevant environment. Matrix Biology Plus, 19-20: 100136. ISSN 2590-0285

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Atkinson, Samuel
Author

Neilson, Lisa J. and Cartwright, Douglas and Risteli, Maija and Jokinen, Elina M. and McGarry, Lynn and Sandvik, Toni and Nikolatou, Konstantina and Hodge, Kelly and Atkinson, Samuel and Vias, Maria and Kay, Emily J. and Brenton, James D. and Carlin, Leo M. and Bryant, David M. and Salo, Tuula and Zanivan, Sara (2023) Omentum-derived matrix enables the study of metastatic ovarian cancer and stromal cell functions in a physiologically relevant environment. Matrix Biology Plus, 19-20: 100136. ISSN 2590-0285

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Vias, Maria
Author

Neilson, Lisa J. and Cartwright, Douglas and Risteli, Maija and Jokinen, Elina M. and McGarry, Lynn and Sandvik, Toni and Nikolatou, Konstantina and Hodge, Kelly and Atkinson, Samuel and Vias, Maria and Kay, Emily J. and Brenton, James D. and Carlin, Leo M. and Bryant, David M. and Salo, Tuula and Zanivan, Sara (2023) Omentum-derived matrix enables the study of metastatic ovarian cancer and stromal cell functions in a physiologically relevant environment. Matrix Biology Plus, 19-20: 100136. ISSN 2590-0285

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Kay, Emily J.
Author

Santi, Alice and Kay, Emily J. and Neilson, Lisa J. and McGarry, Lynn and Lilla, Sergio and Mullin, Margaret and Paul, Nikki R. and Fercoq, Frederic and Koulouras, Grigorios and Rodriguez Blanco, Giovanny and Athineos, Dimitris and Mason, Susan and Hughes, Mark and Thomson, Gemma and Kieffer, Yann and Nixon, Colin and Blyth, Karen and Mechta-Grigoriou, Fatima and Carlin, Leo M. and Zanivan, Sara (2024) Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell function. Science Signaling, 17 (827): eade0580. ISSN 1945-0877

Neilson, Lisa J. and Cartwright, Douglas and Risteli, Maija and Jokinen, Elina M. and McGarry, Lynn and Sandvik, Toni and Nikolatou, Konstantina and Hodge, Kelly and Atkinson, Samuel and Vias, Maria and Kay, Emily J. and Brenton, James D. and Carlin, Leo M. and Bryant, David M. and Salo, Tuula and Zanivan, Sara (2023) Omentum-derived matrix enables the study of metastatic ovarian cancer and stromal cell functions in a physiologically relevant environment. Matrix Biology Plus, 19-20: 100136. ISSN 2590-0285

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Brenton, James D.
Author

Spear, Sarah and Le Saux, Olivia and Mirza, Hasan B. and Iyer, Nayana and Tyson, Katie and Grundland Freile, Fabio and Walton, Josephine B. and Woodman, Chloe and Jarvis, Sheba and Ennis, Darren P. and Aguirre Hernandez, Carmen and Xu, Yuewei and Spiliopoulou, Pavlina and Brenton, James D. and Costa-Pereira, Ana P. and Cook, David P. and Vanderhyden, Barbara C. and Keun, Hector C. and Triantafyllou, Evangelos and Arnold, James N. and McNeish, Iain A. (2024) PTEN loss shapes macrophage dynamics in high grade serous ovarian carcinoma. Cancer Research. ISSN 0008-5472 (In Press)

Neilson, Lisa J. and Cartwright, Douglas and Risteli, Maija and Jokinen, Elina M. and McGarry, Lynn and Sandvik, Toni and Nikolatou, Konstantina and Hodge, Kelly and Atkinson, Samuel and Vias, Maria and Kay, Emily J. and Brenton, James D. and Carlin, Leo M. and Bryant, David M. and Salo, Tuula and Zanivan, Sara (2023) Omentum-derived matrix enables the study of metastatic ovarian cancer and stromal cell functions in a physiologically relevant environment. Matrix Biology Plus, 19-20: 100136. ISSN 2590-0285

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Carlin, Leo M.
Author

Li, Chenxi and Liu, Qiongliang and Han, Lianyong and Zhang, Haiyun and Immler, Roland and Rathkolb, Birgit and Secklehner, Judith and de Angelis, Martin Hrabe and Yildirim, Ali Önder and Zeuschner, Dagmar and Nicke, Annette and Carlin, Leo M. and Sperandio, Markus and Stoeger, Tobias and Rehberg, Markus (2024) The eATP/P2×7R Axis drives quantum dot-nanoparticle induced neutrophil recruitment in the pulmonary microcirculation. Advanced Science, 11 (45): 2404661. ISSN 2198-3844

Xavier, Vanessa and Martinelli, Silvia and Corbyn, Ryan and Pennie, Rachel and Rakovic, Kai and Powley, Ian R. and Officer-Jones, Leah and Ruscica, Vincenzo and Galloway, Alison and Carlin, Leo M. and Cowling, Victoria H. and Le Quesne, John and Martinou, Jean-Claude and MacVicar, Thomas (2024) Mitochondrial double-stranded RNA homeostasis depends on cell-cycle progression. Life Science Alliance, 7 (11): e202402764. ISSN 2575-1077

Santi, Alice and Kay, Emily J. and Neilson, Lisa J. and McGarry, Lynn and Lilla, Sergio and Mullin, Margaret and Paul, Nikki R. and Fercoq, Frederic and Koulouras, Grigorios and Rodriguez Blanco, Giovanny and Athineos, Dimitris and Mason, Susan and Hughes, Mark and Thomson, Gemma and Kieffer, Yann and Nixon, Colin and Blyth, Karen and Mechta-Grigoriou, Fatima and Carlin, Leo M. and Zanivan, Sara (2024) Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell function. Science Signaling, 17 (827): eade0580. ISSN 1945-0877

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Bryant, David M.
Author

Bryant, David M. (2023) We are the system. Journal of Cell Science, 136 (24): jcs261864. ISSN 0021-9533

Neilson, Lisa J. and Cartwright, Douglas and Risteli, Maija and Jokinen, Elina M. and McGarry, Lynn and Sandvik, Toni and Nikolatou, Konstantina and Hodge, Kelly and Atkinson, Samuel and Vias, Maria and Kay, Emily J. and Brenton, James D. and Carlin, Leo M. and Bryant, David M. and Salo, Tuula and Zanivan, Sara (2023) Omentum-derived matrix enables the study of metastatic ovarian cancer and stromal cell functions in a physiologically relevant environment. Matrix Biology Plus, 19-20: 100136. ISSN 2590-0285

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Salo, Tuula
Author

Neilson, Lisa J. and Cartwright, Douglas and Risteli, Maija and Jokinen, Elina M. and McGarry, Lynn and Sandvik, Toni and Nikolatou, Konstantina and Hodge, Kelly and Atkinson, Samuel and Vias, Maria and Kay, Emily J. and Brenton, James D. and Carlin, Leo M. and Bryant, David M. and Salo, Tuula and Zanivan, Sara (2023) Omentum-derived matrix enables the study of metastatic ovarian cancer and stromal cell functions in a physiologically relevant environment. Matrix Biology Plus, 19-20: 100136. ISSN 2590-0285

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Zanivan, Sara
Author

Singh, Shashi P. and Smyth, Danielle J. and Cunningham, Kyle T. and Mukundan, Ananya and Byeon, Chang-Hyeock and Hinck, Cynthia S. and White, Madeleine P.J. and Ciancia, Claire and Wąsowska, Natalia and Sanders, Anna and Jin, Regina and White, Ruby F. and Lilla, Sergio and Zanivan, Sara and Schoenherr, Christina and Inman, Gareth and van Dinther, Maarten and ten Dijke, Peter and Hinck, Andrew P. and Maizels, Rick M. (2025) The TGF-β mimic TGM4 achieves cell specificity through combinatorial surface co-receptor binding. EMBO Reports, 26 (1). pp. 218-244. ISSN 1469-221X

Santi, Alice and Kay, Emily J. and Neilson, Lisa J. and McGarry, Lynn and Lilla, Sergio and Mullin, Margaret and Paul, Nikki R. and Fercoq, Frederic and Koulouras, Grigorios and Rodriguez Blanco, Giovanny and Athineos, Dimitris and Mason, Susan and Hughes, Mark and Thomson, Gemma and Kieffer, Yann and Nixon, Colin and Blyth, Karen and Mechta-Grigoriou, Fatima and Carlin, Leo M. and Zanivan, Sara (2024) Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell function. Science Signaling, 17 (827): eade0580. ISSN 1945-0877

Vringer, Esmee and Heilig, Rosalie and Riley, Joel S. and Black, Annabel and Cloix, Catherine and Skalka, George and Montes-Gómez, Alfredo E. and Aguado, Aurore and Lilla, Sergio and Walczak, Henning and Gyrd-Hansen, Mads and Murphy, Daniel J. and Huang, Danny T. and Zanivan, Sara and Tait, Stephen W.G. (2024) Mitochondrial outer membrane integrity regulates a ubiquitin-dependent and NF-κB-mediated inflammatory response. EMBO Journal, 43 (6). pp. 904-930. ISSN 0261-4189

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