Li, Chenxi and Liu, Qiongliang and Han, Lianyong and Zhang, Haiyun and Immler, Roland and Rathkolb, Birgit and Secklehner, Judith and de Angelis, Martin Hrabe and Yildirim, Ali Önder and Zeuschner, Dagmar and Nicke, Annette and Carlin, Leo M. and Sperandio, Markus and Stoeger, Tobias and Rehberg, Markus (2024) The eATP/P2×7R Axis drives quantum dot-nanoparticle induced neutrophil recruitment in the pulmonary microcirculation. Advanced Science, 11 (45): 2404661. ISSN 2198-3844
AI Summary:
This study investigates the effects of nanoparticles (NPs) on neutrophil recruitment during sterile inflammation. The results show that NPs can trigger rapid neutrophil recruitment, leading to cellular degranulation and release of pro-inflammatory mediators.AI Topics:
Exposure to nanoparticles (NPs) is frequently associated with adverse cardiovascular effects. In contrast, NPs in nanomedicine hold great promise for precise lung-specific drug delivery, especially considering the extensive pulmonary capillary network that facilitates interactions with bloodstream-suspended particles. Therefore, exact knowledge about effects of engineered NPs within the pulmonary microcirculation are instrumental for future application of this technology in patients. To unravel the real-time dynamics of intravenously delivered NPs and their effects in the pulmonary microvasculature, we employed intravital microscopy of the mouse lung. Only PEG-amine-QDs, but not carboxyl-QDs triggered rapid neutrophil recruitment in microvessels and their subsequent recruitment to the alveolar space and was linked to cellular degranulation, TNF-α, and DAMP release into the circulation, particularly eATP. Stimulation of the ATP-gated receptor P2X7R induced expression of E-selectin on microvascular endothelium thereby mediating the neutrophilic immune response. Leukocyte integrins LFA-1 and MAC-1 facilitated adhesion and decelerated neutrophil crawling on the vascular surface. In summary, this study unravels the complex cascade of neutrophil recruitment during NP-induced sterile inflammation. Thereby we demonstrate novel adverse effects for NPs in the pulmonary microcirculation and provide critical insights for optimizing NP-based drug delivery and therapeutic intervention strategies, to ensure their efficacy and safety in clinical applications.
Li, Chenxi
Author
Li, Chenxi and Liu, Qiongliang and Han, Lianyong and Zhang, Haiyun and Immler, Roland and Rathkolb, Birgit and Secklehner, Judith and de Angelis, Martin Hrabe and Yildirim, Ali Önder and Zeuschner, Dagmar and Nicke, Annette and Carlin, Leo M. and Sperandio, Markus and Stoeger, Tobias and Rehberg, Markus (2024) The eATP/P2×7R Axis drives quantum dot-nanoparticle induced neutrophil recruitment in the pulmonary microcirculation. Advanced Science, 11 (45): 2404661. ISSN 2198-3844
See full publications listLiu, Qiongliang
Author
Li, Chenxi and Liu, Qiongliang and Han, Lianyong and Zhang, Haiyun and Immler, Roland and Rathkolb, Birgit and Secklehner, Judith and de Angelis, Martin Hrabe and Yildirim, Ali Önder and Zeuschner, Dagmar and Nicke, Annette and Carlin, Leo M. and Sperandio, Markus and Stoeger, Tobias and Rehberg, Markus (2024) The eATP/P2×7R Axis drives quantum dot-nanoparticle induced neutrophil recruitment in the pulmonary microcirculation. Advanced Science, 11 (45): 2404661. ISSN 2198-3844
See full publications listHan, Lianyong
Author
Li, Chenxi and Liu, Qiongliang and Han, Lianyong and Zhang, Haiyun and Immler, Roland and Rathkolb, Birgit and Secklehner, Judith and de Angelis, Martin Hrabe and Yildirim, Ali Önder and Zeuschner, Dagmar and Nicke, Annette and Carlin, Leo M. and Sperandio, Markus and Stoeger, Tobias and Rehberg, Markus (2024) The eATP/P2×7R Axis drives quantum dot-nanoparticle induced neutrophil recruitment in the pulmonary microcirculation. Advanced Science, 11 (45): 2404661. ISSN 2198-3844
See full publications listZhang, Haiyun
Author
Li, Chenxi and Liu, Qiongliang and Han, Lianyong and Zhang, Haiyun and Immler, Roland and Rathkolb, Birgit and Secklehner, Judith and de Angelis, Martin Hrabe and Yildirim, Ali Önder and Zeuschner, Dagmar and Nicke, Annette and Carlin, Leo M. and Sperandio, Markus and Stoeger, Tobias and Rehberg, Markus (2024) The eATP/P2×7R Axis drives quantum dot-nanoparticle induced neutrophil recruitment in the pulmonary microcirculation. Advanced Science, 11 (45): 2404661. ISSN 2198-3844
See full publications listImmler, Roland
Author
Li, Chenxi and Liu, Qiongliang and Han, Lianyong and Zhang, Haiyun and Immler, Roland and Rathkolb, Birgit and Secklehner, Judith and de Angelis, Martin Hrabe and Yildirim, Ali Önder and Zeuschner, Dagmar and Nicke, Annette and Carlin, Leo M. and Sperandio, Markus and Stoeger, Tobias and Rehberg, Markus (2024) The eATP/P2×7R Axis drives quantum dot-nanoparticle induced neutrophil recruitment in the pulmonary microcirculation. Advanced Science, 11 (45): 2404661. ISSN 2198-3844
See full publications listRathkolb, Birgit
Author
Li, Chenxi and Liu, Qiongliang and Han, Lianyong and Zhang, Haiyun and Immler, Roland and Rathkolb, Birgit and Secklehner, Judith and de Angelis, Martin Hrabe and Yildirim, Ali Önder and Zeuschner, Dagmar and Nicke, Annette and Carlin, Leo M. and Sperandio, Markus and Stoeger, Tobias and Rehberg, Markus (2024) The eATP/P2×7R Axis drives quantum dot-nanoparticle induced neutrophil recruitment in the pulmonary microcirculation. Advanced Science, 11 (45): 2404661. ISSN 2198-3844
See full publications listSecklehner, Judith
Author
Li, Chenxi and Liu, Qiongliang and Han, Lianyong and Zhang, Haiyun and Immler, Roland and Rathkolb, Birgit and Secklehner, Judith and de Angelis, Martin Hrabe and Yildirim, Ali Önder and Zeuschner, Dagmar and Nicke, Annette and Carlin, Leo M. and Sperandio, Markus and Stoeger, Tobias and Rehberg, Markus (2024) The eATP/P2×7R Axis drives quantum dot-nanoparticle induced neutrophil recruitment in the pulmonary microcirculation. Advanced Science, 11 (45): 2404661. ISSN 2198-3844
See full publications listde Angelis, Martin Hrabe
Author
Li, Chenxi and Liu, Qiongliang and Han, Lianyong and Zhang, Haiyun and Immler, Roland and Rathkolb, Birgit and Secklehner, Judith and de Angelis, Martin Hrabe and Yildirim, Ali Önder and Zeuschner, Dagmar and Nicke, Annette and Carlin, Leo M. and Sperandio, Markus and Stoeger, Tobias and Rehberg, Markus (2024) The eATP/P2×7R Axis drives quantum dot-nanoparticle induced neutrophil recruitment in the pulmonary microcirculation. Advanced Science, 11 (45): 2404661. ISSN 2198-3844
See full publications listYildirim, Ali Önder
Author
Li, Chenxi and Liu, Qiongliang and Han, Lianyong and Zhang, Haiyun and Immler, Roland and Rathkolb, Birgit and Secklehner, Judith and de Angelis, Martin Hrabe and Yildirim, Ali Önder and Zeuschner, Dagmar and Nicke, Annette and Carlin, Leo M. and Sperandio, Markus and Stoeger, Tobias and Rehberg, Markus (2024) The eATP/P2×7R Axis drives quantum dot-nanoparticle induced neutrophil recruitment in the pulmonary microcirculation. Advanced Science, 11 (45): 2404661. ISSN 2198-3844
See full publications listZeuschner, Dagmar
Author
Li, Chenxi and Liu, Qiongliang and Han, Lianyong and Zhang, Haiyun and Immler, Roland and Rathkolb, Birgit and Secklehner, Judith and de Angelis, Martin Hrabe and Yildirim, Ali Önder and Zeuschner, Dagmar and Nicke, Annette and Carlin, Leo M. and Sperandio, Markus and Stoeger, Tobias and Rehberg, Markus (2024) The eATP/P2×7R Axis drives quantum dot-nanoparticle induced neutrophil recruitment in the pulmonary microcirculation. Advanced Science, 11 (45): 2404661. ISSN 2198-3844
See full publications listNicke, Annette
Author
Li, Chenxi and Liu, Qiongliang and Han, Lianyong and Zhang, Haiyun and Immler, Roland and Rathkolb, Birgit and Secklehner, Judith and de Angelis, Martin Hrabe and Yildirim, Ali Önder and Zeuschner, Dagmar and Nicke, Annette and Carlin, Leo M. and Sperandio, Markus and Stoeger, Tobias and Rehberg, Markus (2024) The eATP/P2×7R Axis drives quantum dot-nanoparticle induced neutrophil recruitment in the pulmonary microcirculation. Advanced Science, 11 (45): 2404661. ISSN 2198-3844
See full publications listCarlin, Leo M.
Author
Li, Chenxi and Liu, Qiongliang and Han, Lianyong and Zhang, Haiyun and Immler, Roland and Rathkolb, Birgit and Secklehner, Judith and de Angelis, Martin Hrabe and Yildirim, Ali Önder and Zeuschner, Dagmar and Nicke, Annette and Carlin, Leo M. and Sperandio, Markus and Stoeger, Tobias and Rehberg, Markus (2024) The eATP/P2×7R Axis drives quantum dot-nanoparticle induced neutrophil recruitment in the pulmonary microcirculation. Advanced Science, 11 (45): 2404661. ISSN 2198-3844
Xavier, Vanessa and Martinelli, Silvia and Corbyn, Ryan and Pennie, Rachel and Rakovic, Kai and Powley, Ian R. and Officer-Jones, Leah and Ruscica, Vincenzo and Galloway, Alison and Carlin, Leo M. and Cowling, Victoria H. and Le Quesne, John and Martinou, Jean-Claude and MacVicar, Thomas (2024) Mitochondrial double-stranded RNA homeostasis depends on cell-cycle progression. Life Science Alliance, 7 (11): e202402764. ISSN 2575-1077
Santi, Alice and Kay, Emily J. and Neilson, Lisa J. and McGarry, Lynn and Lilla, Sergio and Mullin, Margaret and Paul, Nikki R. and Fercoq, Frederic and Koulouras, Grigorios and Rodriguez Blanco, Giovanny and Athineos, Dimitris and Mason, Susan and Hughes, Mark and Thomson, Gemma and Kieffer, Yann and Nixon, Colin and Blyth, Karen and Mechta-Grigoriou, Fatima and Carlin, Leo M. and Zanivan, Sara (2024) Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell function. Science Signaling, 17 (827): eade0580. ISSN 1945-0877
See full publications listSperandio, Markus
Author
Li, Chenxi and Liu, Qiongliang and Han, Lianyong and Zhang, Haiyun and Immler, Roland and Rathkolb, Birgit and Secklehner, Judith and de Angelis, Martin Hrabe and Yildirim, Ali Önder and Zeuschner, Dagmar and Nicke, Annette and Carlin, Leo M. and Sperandio, Markus and Stoeger, Tobias and Rehberg, Markus (2024) The eATP/P2×7R Axis drives quantum dot-nanoparticle induced neutrophil recruitment in the pulmonary microcirculation. Advanced Science, 11 (45): 2404661. ISSN 2198-3844
See full publications listStoeger, Tobias
Author
Li, Chenxi and Liu, Qiongliang and Han, Lianyong and Zhang, Haiyun and Immler, Roland and Rathkolb, Birgit and Secklehner, Judith and de Angelis, Martin Hrabe and Yildirim, Ali Önder and Zeuschner, Dagmar and Nicke, Annette and Carlin, Leo M. and Sperandio, Markus and Stoeger, Tobias and Rehberg, Markus (2024) The eATP/P2×7R Axis drives quantum dot-nanoparticle induced neutrophil recruitment in the pulmonary microcirculation. Advanced Science, 11 (45): 2404661. ISSN 2198-3844
See full publications listRehberg, Markus
Author
Li, Chenxi and Liu, Qiongliang and Han, Lianyong and Zhang, Haiyun and Immler, Roland and Rathkolb, Birgit and Secklehner, Judith and de Angelis, Martin Hrabe and Yildirim, Ali Önder and Zeuschner, Dagmar and Nicke, Annette and Carlin, Leo M. and Sperandio, Markus and Stoeger, Tobias and Rehberg, Markus (2024) The eATP/P2×7R Axis drives quantum dot-nanoparticle induced neutrophil recruitment in the pulmonary microcirculation. Advanced Science, 11 (45): 2404661. ISSN 2198-3844
See full publications listAvailable under License Creative Commons Attribution.
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