Badar, Ayesha and Aqueel, Rhea and Nawaz, Ali and Ijaz, Umer Zeeshan and Malik, Kauser Abdulla (2025) Microbiota transplantation for Cotton Leaf Curl Disease suppression - core microbiome and transcriptome dynamics. Communications Biology, 8 (1): 380. ISSN 2399-3642
AI Summary:
Microbiota transplantation from Gossypium arboreum FDH228 to Gossypium hirsutum varieties significantly suppresses Cotton Leaf Curl Disease (CLCuD) incidence. The study highlights specific microbial and genetic mechanisms contributing to CLCuD resistance.AI Topics:
Microbiota transplantation is a strong tool for managing plant disease. This study investigates the effects of microbiota transplantation on Cotton Leaf Curl Disease (CLCuD) resistance in Gossypium hirsutum, a species with good fiber length but high susceptibility to biotic stresses. Using metabarcoding for V3-V4 16S rRNA gene amplicon, microbial fractions from both rhizosphere and phyllosphere of CLCuD-resistant species Gossypium arboreum, and susceptible cotton varieties are analyzed. Unique bacterial taxa have been identified associated with disease resistance. Interspecies and intraspecies microbiota transplantation is conducted, followed by CLCuD incidence assays. It is seen that rhizospheric microbiota transplantation from G. arboreum FDH228 significantly suppresses CLCuD in G. hirsutum varieties, outperforming exogenous salicylic acid application. While phyllospheric transplants also reduce disease incidence, they are less effective than rhizospheric transplants. Differential expression analysis DESeq2 is utilized to identify key bacterial genera correlated with CLCuD suppression, including Pseudoxanthomonas and Stenotrophomonas in the rhizosphere of G. arboreum FDH228. Functional pathway analysis reveals upregulation of stress response and metabolism in tolerant species. Transcriptomics reveals upregulation of genes involved in protein phosphorylation and stress response in interspecies rhizospheric microbiota transplants. This study highlights microbiota transplantation as a sustainable method for controlling CLCuD along with specific microbial and genetic mechanisms contributing to CLCuD resistance.
Badar, Ayesha
Author
Badar, Ayesha and Aqueel, Rhea and Nawaz, Ali and Ijaz, Umer Zeeshan and Malik, Kauser Abdulla (2025) Microbiota transplantation for Cotton Leaf Curl Disease suppression - core microbiome and transcriptome dynamics. Communications Biology, 8 (1): 380. ISSN 2399-3642
Aqueel, Rhea and Badar, Ayesha and Roy, Nazish and Ijaz, Umer Zeeshan and Malik, Kauser Abdulla (2024) Disease resistance correlates with core microbiome diversity in cotton. Current Microbiology, 81 (9): 302. ISSN 0343-8651
Aqueel, Rhea and Badar, Ayesha and Ijaz, Umer and Malik, Abdulla Kauser (2024) Microbial influencers and Cotton Leaf Curl Disease (CLCuD) susceptibility: a network perspective. Frontiers in Microbiology, 15: 1381883. ISSN 1664-302X
See full publications listAqueel, Rhea
Author
Badar, Ayesha and Aqueel, Rhea and Nawaz, Ali and Ijaz, Umer Zeeshan and Malik, Kauser Abdulla (2025) Microbiota transplantation for Cotton Leaf Curl Disease suppression - core microbiome and transcriptome dynamics. Communications Biology, 8 (1): 380. ISSN 2399-3642
Aqueel, Rhea and Badar, Ayesha and Roy, Nazish and Ijaz, Umer Zeeshan and Malik, Kauser Abdulla (2024) Disease resistance correlates with core microbiome diversity in cotton. Current Microbiology, 81 (9): 302. ISSN 0343-8651
Aqueel, Rhea and Badar, Ayesha and Ijaz, Umer and Malik, Abdulla Kauser (2024) Microbial influencers and Cotton Leaf Curl Disease (CLCuD) susceptibility: a network perspective. Frontiers in Microbiology, 15: 1381883. ISSN 1664-302X
See full publications listNawaz, Ali
Author
Badar, Ayesha and Aqueel, Rhea and Nawaz, Ali and Ijaz, Umer Zeeshan and Malik, Kauser Abdulla (2025) Microbiota transplantation for Cotton Leaf Curl Disease suppression - core microbiome and transcriptome dynamics. Communications Biology, 8 (1): 380. ISSN 2399-3642
See full publications listIjaz, Umer
Author
Cholet, Fabien and Vignola, Marta and Quinn, Dominic and Ijaz, Umer Z. and Sloan, William T. and Smith, Cindy J. (2025) Microbial ecology of drinking water biofiltration based on 16S rRNA sequencing: a meta-analysis. Water Research, 281: 123684. ISSN 0043-1354
Saleem, Farrukh and Ameer, Aqsa and Afzal, Farhan and Usman, Muhammad and Irshad, Hamid and Sattar, Sadia and Ijaz, Umer Zeeshan and Javed, Sundus (2025) A cross-sectional survey of poultry management systems, practices and antimicrobial use in relation to disease outbreak in Pakistan. BMC Research Notes, 18: 144. ISSN 1756-0500
Badar, Ayesha and Aqueel, Rhea and Nawaz, Ali and Ijaz, Umer Zeeshan and Malik, Kauser Abdulla (2025) Microbiota transplantation for Cotton Leaf Curl Disease suppression - core microbiome and transcriptome dynamics. Communications Biology, 8 (1): 380. ISSN 2399-3642
See full publications listMalik, Kauser Abdulla
Author
Badar, Ayesha and Aqueel, Rhea and Nawaz, Ali and Ijaz, Umer Zeeshan and Malik, Kauser Abdulla (2025) Microbiota transplantation for Cotton Leaf Curl Disease suppression - core microbiome and transcriptome dynamics. Communications Biology, 8 (1): 380. ISSN 2399-3642
Aqueel, Rhea and Badar, Ayesha and Roy, Nazish and Ijaz, Umer Zeeshan and Malik, Kauser Abdulla (2024) Disease resistance correlates with core microbiome diversity in cotton. Current Microbiology, 81 (9): 302. ISSN 0343-8651
Aqueel, Rhea and Badar, Ayesha and Roy, Nazish and Mushtaq, Qandeel and Ali, Aimen Fatima and Bashir, Aftab and Ijaz, Umer Zeeshan and Malik, Kauser Abdulla (2023) Cotton microbiome profiling and Cotton Leaf Curl Disease (CLCuD) suppression through microbial consortia associated. npj Biofilms and Microbiomes, 9 (1): 100. ISSN 2055-5008
See full publications listAvailable under License Creative Commons Attribution.
Download (6MB) | Preview
 |