Trego, Anna and Palmeiro-Sánchez, Tania and Graham, Alison and Ijaz, Umer Zeeshan and O'Flaherty, Vincent (2024) First evidence for temperature influencing the enrichment, assembly and activity of polyhydroxyalkanoate-synthesizing mixed microbial communities. Frontiers in Systems Biology, 4: 1375472. ISSN 2674-0702
AI Summary:
Three aerobic sequencing batch reactors were operated at different temperatures (15°C, 35°C, and 48°C) to study PHA accumulation. The results showed that all temperatures yielded highly enriched PHA-accumulating consortia.AI Topics:
Polyhydroxyalkanoates (PHA) are popular biopolymers due to their potential use as biodegradable thermoplastics. In this study, three aerobic sequencing batch reactors were operated identically except for their temperatures, which were set at 15 °C, 35 °C, and 48 °C. The reactors were subjected to a feast–famine feeding regime, where carbon sources are supplied intermittently, to enrich PHA-accumulating microbial consortia. The biomass was sampled for 16S rRNA gene amplicon sequencing of both DNA (during the enrichment phase) and cDNA (during the enrichment and accumulation phases). All temperatures yielded highly enriched PHA-accumulating consortia. Thermophilic communities were significantly less diverse than those at low or mesophilic temperatures. In particular, Thauera was highly adaptable, abundant, and active at all temperatures. Low temperatures resulted in reduced PHA production rates and yields. Analysis of the microbial community revealed a collapse of community diversity during low-temperature PHA accumulation, suggesting that the substrate dosing strategy was unsuccessful at low temperatures. This points to future possibilities for optimizing low-temperature PHA accumulation.
Trego, Anna
Author
Trego, Anna and O'Sullivan, Sarah and O'Flaherty, Vincent and Collins, Gavin and Ijaz, Umer Zeeshan (2024) Individual methanogenic granules are whole-ecosystem replicates with reproducible responses to environmental cues. Environmental Microbiome, 19: 68. ISSN 2524-6372
Trego, Anna and Palmeiro-Sánchez, Tania and Graham, Alison and Ijaz, Umer Zeeshan and O'Flaherty, Vincent (2024) First evidence for temperature influencing the enrichment, assembly and activity of polyhydroxyalkanoate-synthesizing mixed microbial communities. Frontiers in Systems Biology, 4: 1375472. ISSN 2674-0702
See full publications listPalmeiro-Sánchez, Tania
Author
Trego, Anna and Palmeiro-Sánchez, Tania and Graham, Alison and Ijaz, Umer Zeeshan and O'Flaherty, Vincent (2024) First evidence for temperature influencing the enrichment, assembly and activity of polyhydroxyalkanoate-synthesizing mixed microbial communities. Frontiers in Systems Biology, 4: 1375472. ISSN 2674-0702
See full publications listGraham, Alison
Author
Trego, Anna and Palmeiro-Sánchez, Tania and Graham, Alison and Ijaz, Umer Zeeshan and O'Flaherty, Vincent (2024) First evidence for temperature influencing the enrichment, assembly and activity of polyhydroxyalkanoate-synthesizing mixed microbial communities. Frontiers in Systems Biology, 4: 1375472. ISSN 2674-0702
See full publications listIjaz, Umer
Author
Cholet, Fabien and Vignola, Marta and Quinn, Dominic and Ijaz, Umer Z. and Sloan, William T. and Smith, Cindy J. (2025) Microbial ecology of drinking water biofiltration based on 16S rRNA sequencing: a meta-analysis. Water Research, 281: 123684. ISSN 0043-1354
Saleem, Farrukh and Ameer, Aqsa and Afzal, Farhan and Usman, Muhammad and Irshad, Hamid and Sattar, Sadia and Ijaz, Umer Zeeshan and Javed, Sundus (2025) A cross-sectional survey of poultry management systems, practices and antimicrobial use in relation to disease outbreak in Pakistan. BMC Research Notes, 18: 144. ISSN 1756-0500
Badar, Ayesha and Aqueel, Rhea and Nawaz, Ali and Ijaz, Umer Zeeshan and Malik, Kauser Abdulla (2025) Microbiota transplantation for Cotton Leaf Curl Disease suppression - core microbiome and transcriptome dynamics. Communications Biology, 8 (1): 380. ISSN 2399-3642
See full publications listO'Flaherty, Vincent
Author
Trego, Anna and O'Sullivan, Sarah and O'Flaherty, Vincent and Collins, Gavin and Ijaz, Umer Zeeshan (2024) Individual methanogenic granules are whole-ecosystem replicates with reproducible responses to environmental cues. Environmental Microbiome, 19: 68. ISSN 2524-6372
Trego, Anna and Palmeiro-Sánchez, Tania and Graham, Alison and Ijaz, Umer Zeeshan and O'Flaherty, Vincent (2024) First evidence for temperature influencing the enrichment, assembly and activity of polyhydroxyalkanoate-synthesizing mixed microbial communities. Frontiers in Systems Biology, 4: 1375472. ISSN 2674-0702
Dessì, Paolo and Buenaño, Claribel and Martínez-Sosa, Santiago and Mills, Simon and Trego, Anna and Ijaz, Umer Z. and Pant, Deepak and Puig, Sebastià and O'Flaherty, Vincent and Farràs, Pau (2023) Microbial electrosynthesis of acetate from CO2 in three-chamber cells with gas diffusion biocathode under moderate saline conditions. Environmental Science and Ecotechnology, 16: 100261. ISSN 2666-4984
See full publications listAvailable under License Creative Commons Attribution.
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