Trego, Anna and O'Sullivan, Sarah and O'Flaherty, Vincent and Collins, Gavin and Ijaz, Umer Zeeshan (2024) Individual methanogenic granules are whole-ecosystem replicates with reproducible responses to environmental cues. Environmental Microbiome, 19: 68. ISSN 2524-6372
AI Summary:
Individual anaerobic granules were used as true community replicates to assess whole-microbial-community responses to environmental cues. The results showed that single anaerobic granules can be considered highly-replicated whole-ecosystems with potential usefulness for the field of microbial ecology.AI Topics:
Background
In this study, individual methanogenic (anaerobic), granular biofilms were used as true community replicates to assess whole-microbial-community responses to environmental cues. The aggregates were sourced from a lab-scale, engineered, biological wastewater treatment system, were size-separated, and the largest granules were individually subjected to controlled environmental cues in micro-batch reactors (μBRs).
Results
Individual granules were identical with respect to the structure of the active community based on cDNA analysis. Additionally, it was observed that the active microbial community of individual granules, at the depth of 16S rRNA gene sequencing, produced reproducible responses to environmental changes in pH, temperature, substrate, and trace-metal supplementation. We identified resilient and susceptible taxa associated with each environmental condition tested, as well as selected specialists, whose niche preferences span the entire trophic chain required for the complete anaerobic degradation of organic matter.
Conclusions
We found that single anaerobic granules can be considered highly-replicated whole-ecosystems with potential usefulness for the field of microbial ecology. Additionally, they act as the smallest whole-community unit within the meta-community of an engineered bioreactor. When subjected to various environmental cues, anaerobic granules responded reproducibly allowing for rare or unique opportunities for high-throughput studies testing whole-community responses to a wide range of environmental conditions.
Trego, Anna
Author
Trego, Anna and O'Sullivan, Sarah and O'Flaherty, Vincent and Collins, Gavin and Ijaz, Umer Zeeshan (2024) Individual methanogenic granules are whole-ecosystem replicates with reproducible responses to environmental cues. Environmental Microbiome, 19: 68. ISSN 2524-6372
Trego, Anna and Palmeiro-Sánchez, Tania and Graham, Alison and Ijaz, Umer Zeeshan and O'Flaherty, Vincent (2024) First evidence for temperature influencing the enrichment, assembly and activity of polyhydroxyalkanoate-synthesizing mixed microbial communities. Frontiers in Systems Biology, 4: 1375472. ISSN 2674-0702
See full publications listO'Sullivan, Sarah
Author
Trego, Anna and O'Sullivan, Sarah and O'Flaherty, Vincent and Collins, Gavin and Ijaz, Umer Zeeshan (2024) Individual methanogenic granules are whole-ecosystem replicates with reproducible responses to environmental cues. Environmental Microbiome, 19: 68. ISSN 2524-6372
See full publications listO'Flaherty, Vincent
Author
Trego, Anna and O'Sullivan, Sarah and O'Flaherty, Vincent and Collins, Gavin and Ijaz, Umer Zeeshan (2024) Individual methanogenic granules are whole-ecosystem replicates with reproducible responses to environmental cues. Environmental Microbiome, 19: 68. ISSN 2524-6372
Trego, Anna and Palmeiro-Sánchez, Tania and Graham, Alison and Ijaz, Umer Zeeshan and O'Flaherty, Vincent (2024) First evidence for temperature influencing the enrichment, assembly and activity of polyhydroxyalkanoate-synthesizing mixed microbial communities. Frontiers in Systems Biology, 4: 1375472. ISSN 2674-0702
Dessì, Paolo and Buenaño, Claribel and Martínez-Sosa, Santiago and Mills, Simon and Trego, Anna and Ijaz, Umer Z. and Pant, Deepak and Puig, Sebastià and O'Flaherty, Vincent and Farràs, Pau (2023) Microbial electrosynthesis of acetate from CO2 in three-chamber cells with gas diffusion biocathode under moderate saline conditions. Environmental Science and Ecotechnology, 16: 100261. ISSN 2666-4984
See full publications listCollins, Gavin
Author
Trego, Anna and O'Sullivan, Sarah and O'Flaherty, Vincent and Collins, Gavin and Ijaz, Umer Zeeshan (2024) Individual methanogenic granules are whole-ecosystem replicates with reproducible responses to environmental cues. Environmental Microbiome, 19: 68. ISSN 2524-6372
See full publications listIjaz, Umer
Author
Cholet, Fabien and Vignola, Marta and Quinn, Dominic and Ijaz, Umer Z. and Sloan, William T. and Smith, Cindy J. (2025) Microbial ecology of drinking water biofiltration based on 16S rRNA sequencing: a meta-analysis. Water Research, 281: 123684. ISSN 0043-1354
Saleem, Farrukh and Ameer, Aqsa and Afzal, Farhan and Usman, Muhammad and Irshad, Hamid and Sattar, Sadia and Ijaz, Umer Zeeshan and Javed, Sundus (2025) A cross-sectional survey of poultry management systems, practices and antimicrobial use in relation to disease outbreak in Pakistan. BMC Research Notes, 18: 144. ISSN 1756-0500
Badar, Ayesha and Aqueel, Rhea and Nawaz, Ali and Ijaz, Umer Zeeshan and Malik, Kauser Abdulla (2025) Microbiota transplantation for Cotton Leaf Curl Disease suppression - core microbiome and transcriptome dynamics. Communications Biology, 8 (1): 380. ISSN 2399-3642
See full publications listAvailable under License Creative Commons Attribution.
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