Shi, Xiang and Pereira, Ryan and Uzma and Savage, Laurie and Poursat, Baptiste and Quinn, Dominic and Kostrytsia, Anastasiia and Cholet, Fabien and Smith, Cindy J. and Gauchotte-Lindsay, Caroline and Sloan, William T. and Ijaz, Umer and Vignola, Marta (2024) Microbial stratification and DOM removal in drinking water biofilters: implications for enhanced performance. Water Research, 262: 122053. ISSN 0043-1354
AI Summary:
The study investigated the relationships between microbial community structure and their ability to utilise various dissolved organic carbon (DOC) fractions within a DOC pool. The results showed that the bottom microbial community had lower diversity yet exhibited higher interconnections among its members compared to the top microbial community.AI Topics:
Biofiltration is a low-cost, low-energy technology that employs a biologically activated bed of porous medium to reduce the biodegradable fraction of the dissolved organic matter (DOM) pool in source water, resulting in the production of drinking water. Microbial communities at different bed depths within the biofilter play crucial roles in the degradation and removal of dissolved organic carbon (DOC), ultimately impacting its performance. However, the relationships between the composition of microbial communities inhabiting different biofilter depths and their utilisation of various DOC fractions remain poorly understood. To address this knowledge gap, we conducted an experimental study where microbial communities from the upper (i.e., top 10 cm) and lower (i.e., bottom 10 cm) sections of a 30-cm long laboratory-scale biofilter were recovered. These communities were then individually incubated for 10 days using the same source water as the biofilter influent. Our study revealed that the bottom microbial community exhibited lower diversity yet had a co-occurrence network with a higher degree of interconnections among its members compared to the top microbial community. Moreover, we established a direct correlation between the composition and network structure of the microbial communities and their ability to utilise various DOM compounds within a DOM pool. Interestingly, although the bottom microbial community had only 20% of the total cell abundance compared to the top community at the beginning of the incubation, it utilised and hence removed approximately 60% more total DOC from the DOM pool than the top community. While both communities rapidly utilised labile carbon fractions, such as low-molecular-weight neutrals, the utilisation of more refractory carbon fractions, like high-molecular-weight humic substances with an average molecular weight of more than ca. 1451 g/mol, was exclusive to the bottom microbial community. By employing techniques that capture microbial diversity (i.e., flow cytometry and 16S rRNA amplicon sequencing) and considering the complexities of DOM (i.e., LC-OCD), our study provides novel insights into how microbial community structure could influence the microbial-mediated processes of engineering significance in drinking water production. Finally, our findings could offer the opportunity to improve biofilter performances via engineering interventions that shape the compositions of biofilter microbial communities and enhance their utilisation and removal of DOM, most notably the more classically humified and refractory DOM compound groups.
Shi, Xiang
Author
Shi, Xiang and Pereira, Ryan and Uzma and Savage, Laurie and Poursat, Baptiste and Quinn, Dominic and Kostrytsia, Anastasiia and Cholet, Fabien and Smith, Cindy J. and Gauchotte-Lindsay, Caroline and Sloan, William T. and Ijaz, Umer and Vignola, Marta (2024) Microbial stratification and DOM removal in drinking water biofilters: implications for enhanced performance. Water Research, 262: 122053. ISSN 0043-1354
See full publications listPereira, Ryan
Author
Shi, Xiang and Pereira, Ryan and Uzma and Savage, Laurie and Poursat, Baptiste and Quinn, Dominic and Kostrytsia, Anastasiia and Cholet, Fabien and Smith, Cindy J. and Gauchotte-Lindsay, Caroline and Sloan, William T. and Ijaz, Umer and Vignola, Marta (2024) Microbial stratification and DOM removal in drinking water biofilters: implications for enhanced performance. Water Research, 262: 122053. ISSN 0043-1354
Vignola, Marta and Lenselink, Jeanine and Quinn, Dominic and Ijaz, Umer Zeeshan and Pereira, Ryan and Sloan, William T. and Connelly, Stephanie and Moore, Graeme and Gauchotte-Lindsay, Caroline and Smith, Cindy J. (2023) Differential utilisation of dissolved organic matter compound fractions by different biofilter microbial communities. AQUA: Water Infrastructure, Ecosystems and Society, 72 (10). pp. 1837-1851. ISSN 2709-8028
See full publications listUzma
Author
Zainy, Zainy and Hyder, Muhammad Zeeshan and Uzma, Uzma and Fayyaz, Muhammad and Ijaz, Umer Zeeshan and Farrakh, Sumaira (2025) Comparative transcriptome analysis of resistant, moderately resistant and susceptible wheat near isogenic lines in response to Puccinia striiformis tritici. Journal of Plant Interactions, 20 (1): 2481853. ISSN 1742-9145
Bibi, Sara and Kerbiriou, Caroline and Uzma and Mckirdy, Shona and Kostrytsia, Anastasiia and Rasheed, Hifza and Eqani, Syed Ali Musstjab Akber Shah and Gerasimidis, Konstantinos and Nurulain, Syed Muhammad and Ijaz, Umer Zeeshan (2024) Gut microbiome and function are altered for individuals living in high fluoride concentration areas in Pakistan. Ecotoxicology and Environmental Safety, 284: 116959. ISSN 0147-6513
Shi, Xiang and Pereira, Ryan and Uzma and Savage, Laurie and Poursat, Baptiste and Quinn, Dominic and Kostrytsia, Anastasiia and Cholet, Fabien and Smith, Cindy J. and Gauchotte-Lindsay, Caroline and Sloan, William T. and Ijaz, Umer and Vignola, Marta (2024) Microbial stratification and DOM removal in drinking water biofilters: implications for enhanced performance. Water Research, 262: 122053. ISSN 0043-1354
See full publications listSavage, Laurie
Author
Shi, Xiang and Pereira, Ryan and Uzma and Savage, Laurie and Poursat, Baptiste and Quinn, Dominic and Kostrytsia, Anastasiia and Cholet, Fabien and Smith, Cindy J. and Gauchotte-Lindsay, Caroline and Sloan, William T. and Ijaz, Umer and Vignola, Marta (2024) Microbial stratification and DOM removal in drinking water biofilters: implications for enhanced performance. Water Research, 262: 122053. ISSN 0043-1354
Sperling, Justin R. and Poursat, Baptiste A.J. and Savage, Laurie and Christie, Iain and Cuthill, Calum and Aekbote, Badri and McGuire, Katie and Karimullah, Affar S. and Robbie, Jill and Sloan, William T. and Gauchotte-Lindsay, Caroline and Peveler, William J. and Clark, Alasdair W. (2023) A cross-reactive plasmonic sensing array for drinking water assessment. Environmental Science: Nano, 10 (12). pp. 3500-3508. ISSN 2051-8153
See full publications listPoursat, Baptiste
Author
Poursat, Baptiste A.J. and Rempe, Fleur and Pereira, João and Sutton, Nora B. and Ter Heijne, Annemiek (2024) Unravelling the mechanisms of organic micropollutant removal in bio-electrochemical systems: Insights into sorption, electrochemical degradation, and biodegradation processes. Science of the Total Environment, 945: 173932. ISSN 0048-9697
Shi, Xiang and Pereira, Ryan and Uzma and Savage, Laurie and Poursat, Baptiste and Quinn, Dominic and Kostrytsia, Anastasiia and Cholet, Fabien and Smith, Cindy J. and Gauchotte-Lindsay, Caroline and Sloan, William T. and Ijaz, Umer and Vignola, Marta (2024) Microbial stratification and DOM removal in drinking water biofilters: implications for enhanced performance. Water Research, 262: 122053. ISSN 0043-1354
Poursat, Baptiste A.J. and Langenhoff, Alette A.M. and Feng, Jiahao and Goense, Julianne and Peters, Ruud J.B. and Sutton, Nora B. (2024) Effect of ultra-high-density polyethylene microplastic on the sorption and biodegradation of organic micropollutants. Ecotoxicology and Environmental Safety, 279: 116510. ISSN 0147-6513
See full publications listQuinn, Dominic
Author
Shi, Xiang and Pereira, Ryan and Uzma and Savage, Laurie and Poursat, Baptiste and Quinn, Dominic and Kostrytsia, Anastasiia and Cholet, Fabien and Smith, Cindy J. and Gauchotte-Lindsay, Caroline and Sloan, William T. and Ijaz, Umer and Vignola, Marta (2024) Microbial stratification and DOM removal in drinking water biofilters: implications for enhanced performance. Water Research, 262: 122053. ISSN 0043-1354
See full publications listKostrytsia, Anastasiia
Author
Bibi, Sara and Kerbiriou, Caroline and Uzma and Mckirdy, Shona and Kostrytsia, Anastasiia and Rasheed, Hifza and Eqani, Syed Ali Musstjab Akber Shah and Gerasimidis, Konstantinos and Nurulain, Syed Muhammad and Ijaz, Umer Zeeshan (2024) Gut microbiome and function are altered for individuals living in high fluoride concentration areas in Pakistan. Ecotoxicology and Environmental Safety, 284: 116959. ISSN 0147-6513
Shi, Xiang and Pereira, Ryan and Uzma and Savage, Laurie and Poursat, Baptiste and Quinn, Dominic and Kostrytsia, Anastasiia and Cholet, Fabien and Smith, Cindy J. and Gauchotte-Lindsay, Caroline and Sloan, William T. and Ijaz, Umer and Vignola, Marta (2024) Microbial stratification and DOM removal in drinking water biofilters: implications for enhanced performance. Water Research, 262: 122053. ISSN 0043-1354
See full publications listCholet, Fabien
Author
Cholet, Fabien and Vignola, Marta and Quinn, Dominic and Ijaz, Umer Z. and Sloan, William T. and Smith, Cindy J. (2025) Microbial ecology of drinking water biofiltration based on 16S rRNA sequencing: a meta-analysis. Water Research, 281: 123684. ISSN 0043-1354
Cholet, Fabien and Agogué, Hélène and Ijaz, Umer Z. and Lachaussée, Nicolas and Pineau, Philippe and Smith, Cindy J. (2024) Low-abundant but highly transcriptionally active uncharacterised Nitrosomonas drive ammonia-oxidation in the Brouage mudflat, France. Science of the Total Environment, 946: 174312. ISSN 0048-9697
Shi, Xiang and Pereira, Ryan and Uzma and Savage, Laurie and Poursat, Baptiste and Quinn, Dominic and Kostrytsia, Anastasiia and Cholet, Fabien and Smith, Cindy J. and Gauchotte-Lindsay, Caroline and Sloan, William T. and Ijaz, Umer and Vignola, Marta (2024) Microbial stratification and DOM removal in drinking water biofilters: implications for enhanced performance. Water Research, 262: 122053. ISSN 0043-1354
See full publications listSmith, Cindy
Author
Cholet, Fabien and Vignola, Marta and Quinn, Dominic and Ijaz, Umer Z. and Sloan, William T. and Smith, Cindy J. (2025) Microbial ecology of drinking water biofiltration based on 16S rRNA sequencing: a meta-analysis. Water Research, 281: 123684. ISSN 0043-1354
Fantinelli Franco, Fabiane and Malik, Muhammad and Manjakkal, Libu and Roshanghias, Ali and Smith, Cindy and Gauchotte-Lindsay, Caroline (2024) Optimising carbon structures in laser-induced graphene electrodes using design of experiments for enhanced electrochemical sensing characteristics. ACS Applied Materials and Interfaces, 16 (47). pp. 65489-65502. ISSN 1944-8244
Fantinelli Franco, Fabiane and Malik, Muhammad Hassan and Manjakkal, Libu and Roshanghias, Ali and Smith, Cindy J. and Gauchotte-Lindsay, Caroline (2024) Optimizing carbon structures in laser-induced graphene electrodes using design of experiments for enhanced electrochemical sensing characteristics. ACS Applied Materials and Interfaces, 16 (47). pp. 65489-65502. ISSN 1944-8244
See full publications listGauchotte-Lindsay, Caroline
Author
Comadran-Casas, Carla and Unluer, Cise and Bass, Adrian M. and Macdonald, John and Khaksar Najafi, Elmira and Spruzeniece, Liene and Gauchotte-Lindsay, Caroline (2025) Bioremediation of multiple heavy metals through biostimulation of Microbial-Induced Calcite Precipitation at varying calcium-to-urea concentrations. Journal of Hazardous Materials, 491: 137691. ISSN 0304-3894
Fantinelli Franco, Fabiane and Malik, Muhammad and Manjakkal, Libu and Roshanghias, Ali and Smith, Cindy and Gauchotte-Lindsay, Caroline (2024) Optimising carbon structures in laser-induced graphene electrodes using design of experiments for enhanced electrochemical sensing characteristics. ACS Applied Materials and Interfaces, 16 (47). pp. 65489-65502. ISSN 1944-8244
Fantinelli Franco, Fabiane and Malik, Muhammad Hassan and Manjakkal, Libu and Roshanghias, Ali and Smith, Cindy J. and Gauchotte-Lindsay, Caroline (2024) Optimizing carbon structures in laser-induced graphene electrodes using design of experiments for enhanced electrochemical sensing characteristics. ACS Applied Materials and Interfaces, 16 (47). pp. 65489-65502. ISSN 1944-8244
See full publications listSloan, William
Author
Cholet, Fabien and Vignola, Marta and Quinn, Dominic and Ijaz, Umer Z. and Sloan, William T. and Smith, Cindy J. (2025) Microbial ecology of drinking water biofiltration based on 16S rRNA sequencing: a meta-analysis. Water Research, 281: 123684. ISSN 0043-1354
Gupta, Rohit and Murray, Cameron and Sloan, William T. and You, Siming (2025) Predicting the methane production of microwave-pretreated anaerobic digestion of food waste: a machine learning approach. Energy. ISSN 0360-5442 (In Press)
Alghafli, Khaled and Shi, Xiaogang and Sloan, William and Ali, Awad M. (2025) Investigating the role of ENSO in groundwater temporal variability across Abu Dhabi Emirate, United Arab Emirates using machine learning algorithms. Groundwater for Sustainable Development, 28: 101389. ISSN 2352-801X
See full publications listIjaz, Umer
Author
Cholet, Fabien and Vignola, Marta and Quinn, Dominic and Ijaz, Umer Z. and Sloan, William T. and Smith, Cindy J. (2025) Microbial ecology of drinking water biofiltration based on 16S rRNA sequencing: a meta-analysis. Water Research, 281: 123684. ISSN 0043-1354
Saleem, Farrukh and Ameer, Aqsa and Afzal, Farhan and Usman, Muhammad and Irshad, Hamid and Sattar, Sadia and Ijaz, Umer Zeeshan and Javed, Sundus (2025) A cross-sectional survey of poultry management systems, practices and antimicrobial use in relation to disease outbreak in Pakistan. BMC Research Notes, 18: 144. ISSN 1756-0500
Badar, Ayesha and Aqueel, Rhea and Nawaz, Ali and Ijaz, Umer Zeeshan and Malik, Kauser Abdulla (2025) Microbiota transplantation for Cotton Leaf Curl Disease suppression - core microbiome and transcriptome dynamics. Communications Biology, 8 (1): 380. ISSN 2399-3642
See full publications listVignola, Marta
Author
Cholet, Fabien and Vignola, Marta and Quinn, Dominic and Ijaz, Umer Z. and Sloan, William T. and Smith, Cindy J. (2025) Microbial ecology of drinking water biofiltration based on 16S rRNA sequencing: a meta-analysis. Water Research, 281: 123684. ISSN 0043-1354
Shi, Xiang and Pereira, Ryan and Uzma and Savage, Laurie and Poursat, Baptiste and Quinn, Dominic and Kostrytsia, Anastasiia and Cholet, Fabien and Smith, Cindy J. and Gauchotte-Lindsay, Caroline and Sloan, William T. and Ijaz, Umer and Vignola, Marta (2024) Microbial stratification and DOM removal in drinking water biofilters: implications for enhanced performance. Water Research, 262: 122053. ISSN 0043-1354
Vignola, Marta and Lenselink, Jeanine and Quinn, Dominic and Ijaz, Umer Zeeshan and Pereira, Ryan and Sloan, William T. and Connelly, Stephanie and Moore, Graeme and Gauchotte-Lindsay, Caroline and Smith, Cindy J. (2023) Differential utilisation of dissolved organic matter compound fractions by different biofilter microbial communities. AQUA: Water Infrastructure, Ecosystems and Society, 72 (10). pp. 1837-1851. ISSN 2709-8028
See full publications listAvailable under License Creative Commons Attribution.
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