Ashraf, Hajra and Dikarlo, Plamena and Masia, Aurora and Zarbo, Ignazio R. and Solla, Paolo and Ijaz, Umer Zeeshan and Sechi, Leonardo A. (2024) Mycobacterium avium subspecies paratuberculosis (MAP) infection, and its impact on gut microbiome of individuals with Multiple Sclerosis. Scientific Reports, 14: 24027. ISSN 2045-2322
AI Summary:
The study characterized the compositional and functional differences of the gut microbiome between MS patients with and without MAP infection. The results suggest that the gut microbiome may be an important target for dietary and lifestyle intervention in MS patients.AI Topics:
The microbial ecology of Mycobacterium avium subspecies paratuberculosis infections (MAP) within the context of Multiple Sclerosis (MS) is largely an unexplored topic in the literature. Thus, we have characterized the compositional and predicted functional differences of the gut microbiome between MS patients with MAP (MAP+) and without (MAP−) infection. This was done in the context of exposome differences (through self-reported filled questionnaires), principally in anthropometric and sociodemographic patterns to gain an understanding of the gut microbiome dynamics. 16S rRNA microbiome profiling of faecal samples (n = 69) was performed for four groups, which differed by disease and MAP infection: healthy cohort (HC) MAP−; HC MAP+ ; MS MAP−; and MS MAP+ . Using a dynamic strategy, with MAP infection and time of sampling as occupancy models, we have recovered the core microbiome for both HC and MS individuals. Additional application of neutral modeling suggests key genera that are under selection pressure by the hosts. These include members of the phyla Actinobacteriota, Bacteroidota, and Firmicutes. As several subjects provided multiple samples, a Quasi Conditional Association Test that incorporates paired-nature of samples found major differences in Archaea. To consolidate treatment groups, confounders, microbiome, and the disease outcome parameters, a mediation analysis is performed for MS cohort. This highlighted certain genera i.e., Sutterella, Akkermansia, Bacteriodes, Gastranaerophilales, Alistipes, Balutia, Faecalibacterium, Lachnospiraceae, Anaerostipes, Ruminococcaceae, Eggerthellaceae and Clostridia-UCG-014 having mediatory effect using disease duration as an outcome and MAP infection as a treatment group. Our analyses indicate that the gut microbiome may be an important target for dietary and lifestyle intervention in MS patients with and without MAP infection.
Ashraf, Hajra
Author
Ashraf, Hajra and Dikarlo, Plamena and Masia, Aurora and Zarbo, Ignazio R. and Solla, Paolo and Ijaz, Umer Zeeshan and Sechi, Leonardo A. (2024) Mycobacterium avium subspecies paratuberculosis (MAP) infection, and its impact on gut microbiome of individuals with Multiple Sclerosis. Scientific Reports, 14: 24027. ISSN 2045-2322
Ashraf, Hajra and Dikarlo, Plamena and Masia, Aurora and Zarbo, Ignazio R. and Solla, Paolo and Ijaz, Umer Zeeshan and Sechi, Leonardo A. (2024) Network analysis of gut microbial communities reveal key genera for a multiple sclerosis cohort with Mycobacterium avium subspecies paratuberculosis infection. Gut Pathogens, 16 (1): 37. ISSN 1757-4749
See full publications listDikarlo, Plamena
Author
Ashraf, Hajra and Dikarlo, Plamena and Masia, Aurora and Zarbo, Ignazio R. and Solla, Paolo and Ijaz, Umer Zeeshan and Sechi, Leonardo A. (2024) Mycobacterium avium subspecies paratuberculosis (MAP) infection, and its impact on gut microbiome of individuals with Multiple Sclerosis. Scientific Reports, 14: 24027. ISSN 2045-2322
Ashraf, Hajra and Dikarlo, Plamena and Masia, Aurora and Zarbo, Ignazio R. and Solla, Paolo and Ijaz, Umer Zeeshan and Sechi, Leonardo A. (2024) Network analysis of gut microbial communities reveal key genera for a multiple sclerosis cohort with Mycobacterium avium subspecies paratuberculosis infection. Gut Pathogens, 16 (1): 37. ISSN 1757-4749
See full publications listMasia, Aurora
Author
Ashraf, Hajra and Dikarlo, Plamena and Masia, Aurora and Zarbo, Ignazio R. and Solla, Paolo and Ijaz, Umer Zeeshan and Sechi, Leonardo A. (2024) Mycobacterium avium subspecies paratuberculosis (MAP) infection, and its impact on gut microbiome of individuals with Multiple Sclerosis. Scientific Reports, 14: 24027. ISSN 2045-2322
Ashraf, Hajra and Dikarlo, Plamena and Masia, Aurora and Zarbo, Ignazio R. and Solla, Paolo and Ijaz, Umer Zeeshan and Sechi, Leonardo A. (2024) Network analysis of gut microbial communities reveal key genera for a multiple sclerosis cohort with Mycobacterium avium subspecies paratuberculosis infection. Gut Pathogens, 16 (1): 37. ISSN 1757-4749
See full publications listZarbo, Ignazio R.
Author
Ashraf, Hajra and Dikarlo, Plamena and Masia, Aurora and Zarbo, Ignazio R. and Solla, Paolo and Ijaz, Umer Zeeshan and Sechi, Leonardo A. (2024) Mycobacterium avium subspecies paratuberculosis (MAP) infection, and its impact on gut microbiome of individuals with Multiple Sclerosis. Scientific Reports, 14: 24027. ISSN 2045-2322
Ashraf, Hajra and Dikarlo, Plamena and Masia, Aurora and Zarbo, Ignazio R. and Solla, Paolo and Ijaz, Umer Zeeshan and Sechi, Leonardo A. (2024) Network analysis of gut microbial communities reveal key genera for a multiple sclerosis cohort with Mycobacterium avium subspecies paratuberculosis infection. Gut Pathogens, 16 (1): 37. ISSN 1757-4749
See full publications listSolla, Paolo
Author
Ashraf, Hajra and Dikarlo, Plamena and Masia, Aurora and Zarbo, Ignazio R. and Solla, Paolo and Ijaz, Umer Zeeshan and Sechi, Leonardo A. (2024) Mycobacterium avium subspecies paratuberculosis (MAP) infection, and its impact on gut microbiome of individuals with Multiple Sclerosis. Scientific Reports, 14: 24027. ISSN 2045-2322
Ashraf, Hajra and Dikarlo, Plamena and Masia, Aurora and Zarbo, Ignazio R. and Solla, Paolo and Ijaz, Umer Zeeshan and Sechi, Leonardo A. (2024) Network analysis of gut microbial communities reveal key genera for a multiple sclerosis cohort with Mycobacterium avium subspecies paratuberculosis infection. Gut Pathogens, 16 (1): 37. ISSN 1757-4749
See full publications listIjaz, Umer
Author
Cholet, Fabien and Vignola, Marta and Quinn, Dominic and Ijaz, Umer Z. and Sloan, William T. and Smith, Cindy J. (2025) Microbial ecology of drinking water biofiltration based on 16S rRNA sequencing: a meta-analysis. Water Research, 281: 123684. ISSN 0043-1354
Saleem, Farrukh and Ameer, Aqsa and Afzal, Farhan and Usman, Muhammad and Irshad, Hamid and Sattar, Sadia and Ijaz, Umer Zeeshan and Javed, Sundus (2025) A cross-sectional survey of poultry management systems, practices and antimicrobial use in relation to disease outbreak in Pakistan. BMC Research Notes, 18: 144. ISSN 1756-0500
Badar, Ayesha and Aqueel, Rhea and Nawaz, Ali and Ijaz, Umer Zeeshan and Malik, Kauser Abdulla (2025) Microbiota transplantation for Cotton Leaf Curl Disease suppression - core microbiome and transcriptome dynamics. Communications Biology, 8 (1): 380. ISSN 2399-3642
See full publications listSechi, Leonardo A.
Author
Ashraf, Hajra and Dikarlo, Plamena and Masia, Aurora and Zarbo, Ignazio R. and Solla, Paolo and Ijaz, Umer Zeeshan and Sechi, Leonardo A. (2024) Mycobacterium avium subspecies paratuberculosis (MAP) infection, and its impact on gut microbiome of individuals with Multiple Sclerosis. Scientific Reports, 14: 24027. ISSN 2045-2322
Ashraf, Hajra and Dikarlo, Plamena and Masia, Aurora and Zarbo, Ignazio R. and Solla, Paolo and Ijaz, Umer Zeeshan and Sechi, Leonardo A. (2024) Network analysis of gut microbial communities reveal key genera for a multiple sclerosis cohort with Mycobacterium avium subspecies paratuberculosis infection. Gut Pathogens, 16 (1): 37. ISSN 1757-4749
See full publications list
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