Delaney, Christopher and Alapati, Susanth and Alshehri, Muhanna and Kubalova, Dominika and Veena, Chandra Lekha Ramalingam and Abusrewil, Sumaya and Short, Bryn and Bradshaw, David and Brown, Jason L. (2023) Investigating the role of Candida albicans as a universal substrate for oral bacteria using a transcriptomic approach: implications for interkingdom biofilm control? APMIS: Journal of Pathology, Microbiology and Immunology, 131 (11). pp. 601-612. ISSN 0903-4641
AI Summary:
Candida albicans was found to support the growth of aerobic and anaerobic bacteria in a biofilm environment. The study identified specific functional enrichment of pathways in the 4-species community, as well as transcriptional profiles unique to the dual-species biofilms.AI Topics:
Candida albicans is frequently identified as a colonizer of the oral cavity in health and has recently been termed a “keystone” commensal due to its role on the bacterial communities. However, the role that C. albicans plays in such interactions is not fully understood. Therefore, this study aimed to identify the relationship between C. albicans and bacteria associated with oral symbiosis and dysbiosis. To do this, we evaluated the ability of C. albicans to support the growth of the aerobic commensal Streptococcus gordonii and the anaerobic pathogens Fusobacterium nucleatum and Porphyromonas gingivalis in the biofilm environment. RNA-Sequencing with the Illumina platform was then utilized to identify C. albicans gene expression and functional pathways involved during such interactions in dual-species and a 4-species biofilm model. Results indicated that C. albicans was capable of supporting growth of all three bacteria, with a significant increase in colony counts of each bacteria in the dual-species biofilm (p < 0.05). We identified specific functional enrichment of pathways in our 4-species community as well as transcriptional profiles unique to the F. nucleatum and S. gordonii dual-species biofilms, indicating a species-specific effect on C. albicans. Candida-related hemin acquisition and heat shock protein mediated processes were unique to the organism following co-culture with anaerobic and aerobic bacteria, respectively, suggestive that such pathways may be feasible options for therapeutic targeting to interfere with these fungal-bacterial interactions. Targeted antifungal therapy may be considered as an option for biofilm destabilization and treatment of complex communities. Moving forward, we propose that further studies must continue to investigate the role of this fungal organism in the context of the interkingdom nature of oral diseases.
Delaney, Christopher
Author
Ware, Alicia and Johnston, William and Delaney, Christopher and Butcher, Mark C. and Ramage, Gordon and Price, Lesley and Butcher, John and Kean, Ryan (2025) Dry surface biofilm formation by Candida auris facilitates persistence and tolerance to sodium hypochlorite. APMIS: Journal of Pathology, Microbiology and Immunology, 133 (4): e70022. ISSN 0903-4641
Alshehri, Muhanna and Alqahtani, Saeed and Murali, Pranitha and Delaney, Christopher and Johnston, William and Brown, Jason L. (2025) Organotypic tissue model systems for investigating host-pathogen interactions in vitro. Journal of Visualized Experiments, 28 (217): e67487. ISSN 1940-087X
Delaney, Christopher and Short, Bryn and Rajendran, Ranjith and Kean, Ryan and Burgess, Karl and Williams, Craig and Munro, Carol A. and Ramage, Gordon (2023) An integrated transcriptomic and metabolomic approach to investigate the heterogeneous Candida albicans biofilm phenotype. Biofilm, 5: 100112. ISSN 2590-2075
See full publications listAlapati, Susanth
Author
Delaney, Christopher and Alapati, Susanth and Alshehri, Muhanna and Kubalova, Dominika and Veena, Chandra Lekha Ramalingam and Abusrewil, Sumaya and Short, Bryn and Bradshaw, David and Brown, Jason L. (2023) Investigating the role of Candida albicans as a universal substrate for oral bacteria using a transcriptomic approach: implications for interkingdom biofilm control? APMIS: Journal of Pathology, Microbiology and Immunology, 131 (11). pp. 601-612. ISSN 0903-4641
See full publications listAlshehri, Muhanna
Author
Delaney, Christopher and Alapati, Susanth and Alshehri, Muhanna and Kubalova, Dominika and Veena, Chandra Lekha Ramalingam and Abusrewil, Sumaya and Short, Bryn and Bradshaw, David and Brown, Jason L. (2023) Investigating the role of Candida albicans as a universal substrate for oral bacteria using a transcriptomic approach: implications for interkingdom biofilm control? APMIS: Journal of Pathology, Microbiology and Immunology, 131 (11). pp. 601-612. ISSN 0903-4641
See full publications listKubalova, Dominika
Author
Delaney, Christopher and Alapati, Susanth and Alshehri, Muhanna and Kubalova, Dominika and Veena, Chandra Lekha Ramalingam and Abusrewil, Sumaya and Short, Bryn and Bradshaw, David and Brown, Jason L. (2023) Investigating the role of Candida albicans as a universal substrate for oral bacteria using a transcriptomic approach: implications for interkingdom biofilm control? APMIS: Journal of Pathology, Microbiology and Immunology, 131 (11). pp. 601-612. ISSN 0903-4641
See full publications listVeena, Chandra Lekha Ramalingam
Author
Delaney, Christopher and Alapati, Susanth and Alshehri, Muhanna and Kubalova, Dominika and Veena, Chandra Lekha Ramalingam and Abusrewil, Sumaya and Short, Bryn and Bradshaw, David and Brown, Jason L. (2023) Investigating the role of Candida albicans as a universal substrate for oral bacteria using a transcriptomic approach: implications for interkingdom biofilm control? APMIS: Journal of Pathology, Microbiology and Immunology, 131 (11). pp. 601-612. ISSN 0903-4641
See full publications listAbusrewil, Sumaya
Author
Delaney, Christopher and Alapati, Susanth and Alshehri, Muhanna and Kubalova, Dominika and Veena, Chandra Lekha Ramalingam and Abusrewil, Sumaya and Short, Bryn and Bradshaw, David and Brown, Jason L. (2023) Investigating the role of Candida albicans as a universal substrate for oral bacteria using a transcriptomic approach: implications for interkingdom biofilm control? APMIS: Journal of Pathology, Microbiology and Immunology, 131 (11). pp. 601-612. ISSN 0903-4641
See full publications listShort, Bryn
Author
Allkja, Jontana and Bakri, Ahmed and Short, Bryn and Gilmour, Andrew and Brown, Jason L. and Bal, Abhijit M. and Newby, Kelly J.M. and Jenkins, Toby and Short, Rob D. and Williams, Craig and Ramage, Gordon (2025) Investigating the prevalence of fungi in diabetic ulcers: an under-recognised contributor to polymicrobial biofilms. APMIS: Journal of Pathology, Microbiology and Immunology, 133 (4): e70025. ISSN 0903-4641
Abduljalil, Hafsa and Alshanta, Om Alkhir and Chougule, Safa and Butcher, Mark and Short, Bryn and Mclean, William and Parry, Neil and O'Keeffe, Joanne and Ramage, Gordon (2025) Lactams exhibit potent antifungal activity against monospecies and multispecies interkingdom biofilms on a novel hydrogel skin model. APMIS: Journal of Pathology, Microbiology and Immunology, 133 (1): e13510. ISSN 0903-4641
Jatkowska, Aleksandra and Gkikas, Konstantinos and Nichols, Ben and Short, Bryn and Rizou, Vasiliki-Konstantina and Kapranos, Panagiotis and Klein Gunnewiek, Jennier and Christina, Edelyn and Svolos, Vaios and Quince, Christopher and Gerasimidis, Konstantinos (2024) Dose-dependent effects of enteral nutrition on the faecal microbiota and short chain fatty acids. Clinical Nutrition, 43 (5). pp. 1200-1207. ISSN 0261-5614
See full publications listBradshaw, David
Author
Delaney, Christopher and Alapati, Susanth and Alshehri, Muhanna and Kubalova, Dominika and Veena, Chandra Lekha Ramalingam and Abusrewil, Sumaya and Short, Bryn and Bradshaw, David and Brown, Jason L. (2023) Investigating the role of Candida albicans as a universal substrate for oral bacteria using a transcriptomic approach: implications for interkingdom biofilm control? APMIS: Journal of Pathology, Microbiology and Immunology, 131 (11). pp. 601-612. ISSN 0903-4641
See full publications listBrown, Jason Lee
Author
Allkja, Jontana and Bakri, Ahmed and Short, Bryn and Gilmour, Andrew and Brown, Jason L. and Bal, Abhijit M. and Newby, Kelly J.M. and Jenkins, Toby and Short, Rob D. and Williams, Craig and Ramage, Gordon (2025) Investigating the prevalence of fungi in diabetic ulcers: an under-recognised contributor to polymicrobial biofilms. APMIS: Journal of Pathology, Microbiology and Immunology, 133 (4): e70025. ISSN 0903-4641
Alshehri, Muhanna and Alqahtani, Saeed and Murali, Pranitha and Delaney, Christopher and Johnston, William and Brown, Jason L. (2025) Organotypic tissue model systems for investigating host-pathogen interactions in vitro. Journal of Visualized Experiments, 28 (217): e67487. ISSN 1940-087X
Baz, Abdullah and Bakri, Ahmed and Butcher, Mark and Short, Bryn and Ghimire, Bhagirath and Gaur, Nishtha and Jenkins, Toby and Short, Robert D. and Riggio, Marcello and Williams, Craig and Ramage, Gordon and Brown, Jason L. (2023) Staphylococcus aureus strains exhibit heterogenous tolerance to direct cold atmospheric plasma therapy. Biofilm, 5: 100123. ISSN 2590-2075
See full publications listAvailable under License Creative Commons Attribution.
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