Da Silva Costa, Ana and Ghouri, Iffath and Johnston, Alexander and Mcglynn, Karen and McNair, Andrew and Bowman, Peter and Malik, Natasha and Hurren, Johanne and Bingelis, Tomas and Dunne, Michael and Smith, Godfrey L. and Kemi, Ole J. (2023) Electrically stimulated in vitro heart cell mimic of acute exercise reveals novel immediate cellular responses to exercise: Reduced contractility and metabolism, but maintained calcium cycling and increased myofilament calcium sensitivity. Cell Biochemistry and Function, 41 (8). pp. 1147-1161. ISSN 0263-6484
AI Summary:
Acute exercise decreased cardiomyocyte fractional shortening, but Ca2 handling and myofilament Ca2 sensitivity compensated for this. Acidosis and reduced energy substrate and mitochondrial ATP generation explained these changes.AI Topics:
Cardiac cellular responses to acute exercise remain undescribed. We present a model for mimicking acute aerobic endurance exercise to freshly isolated cardiomyocytes by evoking exercise-like contractions over prolonged periods of time with trains of electrical twitch stimulations. We then investigated immediate contractile, Ca2+, and metabolic responses to acute exercise in perfused freshly isolated left ventricular rat cardiomyocytes, after a matrix-design optimized protocol and induced a mimic for acute aerobic endurance exercise by trains of prolonged field twitch stimulations. Acute exercise decreased cardiomyocyte fractional shortening 50%–80% (p < .01). This was not explained by changes to intracellular Ca2+ handling (p > .05); rather, we observed a weak insignificant Ca2+ transient increase (p = .11), while myofilament Ca2+ sensitivity increased 20%–70% (p < .05). Acidic pH 6.8 decreased fractional shortening 20%–70% (p < .05) because of 20%–30% decreased Ca2+ transients (p < .05), but no difference occurred between control and acute exercise (p > .05). Addition of 1 or 10 mM La− increased fractional shortening in control (1 mM La−: no difference, p > .05; 10 mM La−: 20%–30%, p < .05) and acute exercise (1 mM La−: 40%–90%, p < .01; 10 mM La−: 50%–100%, p < .01) and rendered acute exercise indifferent from control (p > .05). Intrinsic autofluorescence showed a resting NADstate of 0.59 ± 0.04 and FADstate of 0.17 ± 0.03, while acute exercise decreased NADH/FAD ratio 8% (p < .01), indicating intracellular oxidation. In conclusion, we show a novel approach for studying immediate acute cardiomyocyte responses to aerobic endurance exercise. We find that acute exercise in cardiomyocytes decreases contraction, but Ca2+ handling and myofilament Ca2+ sensitivity compensate for this, while acidosis and reduced energy substrate and mitochondrial ATP generation explain this.
Da Silva Costa, Ana
Author
Da Silva Costa, Ana and Ghouri, Iffath and Johnston, Alexander and Mcglynn, Karen and McNair, Andrew and Bowman, Peter and Malik, Natasha and Hurren, Johanne and Bingelis, Tomas and Dunne, Michael and Smith, Godfrey L. and Kemi, Ole J. (2023) Electrically stimulated in vitro heart cell mimic of acute exercise reveals novel immediate cellular responses to exercise: Reduced contractility and metabolism, but maintained calcium cycling and increased myofilament calcium sensitivity. Cell Biochemistry and Function, 41 (8). pp. 1147-1161. ISSN 0263-6484
See full publications listGhouri, Iffath
Author
Da Silva Costa, Ana and Ghouri, Iffath and Johnston, Alexander and Mcglynn, Karen and McNair, Andrew and Bowman, Peter and Malik, Natasha and Hurren, Johanne and Bingelis, Tomas and Dunne, Michael and Smith, Godfrey L. and Kemi, Ole J. (2023) Electrically stimulated in vitro heart cell mimic of acute exercise reveals novel immediate cellular responses to exercise: Reduced contractility and metabolism, but maintained calcium cycling and increased myofilament calcium sensitivity. Cell Biochemistry and Function, 41 (8). pp. 1147-1161. ISSN 0263-6484
See full publications listJohnston, Alexander
Author
Elbassioni, Ali Ali Mohamed and Raheem, Anmar A. and Song, Jian and Johnston, Alexander S. and Trivett, Cara and Lin, Hong and Zhang, Haobo and Bradley, Ashley and Higgins, Erin and Mooney, Leanne and Koay, Yen Chin and O'Toole, Dylan and Herzyk, Pawel and Nixon, Colin and Blyth, Karen and O'Sullivan, John F. and Lang, Ninian N. and Berry, Colin and Braun, Thomas and Schiattarella, Gabriele G. and Giacca, Mauro and McBride, Martin W. and Nicklin, Stuart A. and Cameron, Ewan R. and Loughrey, Christopher M. and MacDonald, Eilidh A. (2025) Targeting Runx1 protects against heart failure with preserved ejection fraction. bioRxiv.
Da Silva Costa, Ana and Ghouri, Iffath and Johnston, Alexander and Mcglynn, Karen and McNair, Andrew and Bowman, Peter and Malik, Natasha and Hurren, Johanne and Bingelis, Tomas and Dunne, Michael and Smith, Godfrey L. and Kemi, Ole J. (2023) Electrically stimulated in vitro heart cell mimic of acute exercise reveals novel immediate cellular responses to exercise: Reduced contractility and metabolism, but maintained calcium cycling and increased myofilament calcium sensitivity. Cell Biochemistry and Function, 41 (8). pp. 1147-1161. ISSN 0263-6484
See full publications listMcglynn, Karen
Author
Da Silva Costa, Ana and Ghouri, Iffath and Johnston, Alexander and Mcglynn, Karen and McNair, Andrew and Bowman, Peter and Malik, Natasha and Hurren, Johanne and Bingelis, Tomas and Dunne, Michael and Smith, Godfrey L. and Kemi, Ole J. (2023) Electrically stimulated in vitro heart cell mimic of acute exercise reveals novel immediate cellular responses to exercise: Reduced contractility and metabolism, but maintained calcium cycling and increased myofilament calcium sensitivity. Cell Biochemistry and Function, 41 (8). pp. 1147-1161. ISSN 0263-6484
See full publications listMcNair, Andrew
Author
Da Silva Costa, Ana and Ghouri, Iffath and Johnston, Alexander and Mcglynn, Karen and McNair, Andrew and Bowman, Peter and Malik, Natasha and Hurren, Johanne and Bingelis, Tomas and Dunne, Michael and Smith, Godfrey L. and Kemi, Ole J. (2023) Electrically stimulated in vitro heart cell mimic of acute exercise reveals novel immediate cellular responses to exercise: Reduced contractility and metabolism, but maintained calcium cycling and increased myofilament calcium sensitivity. Cell Biochemistry and Function, 41 (8). pp. 1147-1161. ISSN 0263-6484
See full publications listBowman, Peter
Author
Da Silva Costa, Ana and Ghouri, Iffath and Johnston, Alexander and Mcglynn, Karen and McNair, Andrew and Bowman, Peter and Malik, Natasha and Hurren, Johanne and Bingelis, Tomas and Dunne, Michael and Smith, Godfrey L. and Kemi, Ole J. (2023) Electrically stimulated in vitro heart cell mimic of acute exercise reveals novel immediate cellular responses to exercise: Reduced contractility and metabolism, but maintained calcium cycling and increased myofilament calcium sensitivity. Cell Biochemistry and Function, 41 (8). pp. 1147-1161. ISSN 0263-6484
See full publications listMalik, Natasha
Author
Da Silva Costa, Ana and Ghouri, Iffath and Johnston, Alexander and Mcglynn, Karen and McNair, Andrew and Bowman, Peter and Malik, Natasha and Hurren, Johanne and Bingelis, Tomas and Dunne, Michael and Smith, Godfrey L. and Kemi, Ole J. (2023) Electrically stimulated in vitro heart cell mimic of acute exercise reveals novel immediate cellular responses to exercise: Reduced contractility and metabolism, but maintained calcium cycling and increased myofilament calcium sensitivity. Cell Biochemistry and Function, 41 (8). pp. 1147-1161. ISSN 0263-6484
Malik, Natasha and Hay, Jodie and Almuhanna, Hassan N.B. and Dunn, Karen M. and Lees, Jamie and Cassels, Jennifer and Li, Jiatian and Nakagawa, Rinako and Sansom, Owen J. and Michie, Alison M. (2023) mTORC1-selective activation of translation elongation promotes disease progression in chronic lymphocytic leukemia. Leukemia, 37. pp. 2414-2425. ISSN 0887-6924
See full publications listHurren, Johanne
Author
Da Silva Costa, Ana and Ghouri, Iffath and Johnston, Alexander and Mcglynn, Karen and McNair, Andrew and Bowman, Peter and Malik, Natasha and Hurren, Johanne and Bingelis, Tomas and Dunne, Michael and Smith, Godfrey L. and Kemi, Ole J. (2023) Electrically stimulated in vitro heart cell mimic of acute exercise reveals novel immediate cellular responses to exercise: Reduced contractility and metabolism, but maintained calcium cycling and increased myofilament calcium sensitivity. Cell Biochemistry and Function, 41 (8). pp. 1147-1161. ISSN 0263-6484
See full publications listBingelis, Tomas
Author
Da Silva Costa, Ana and Ghouri, Iffath and Johnston, Alexander and Mcglynn, Karen and McNair, Andrew and Bowman, Peter and Malik, Natasha and Hurren, Johanne and Bingelis, Tomas and Dunne, Michael and Smith, Godfrey L. and Kemi, Ole J. (2023) Electrically stimulated in vitro heart cell mimic of acute exercise reveals novel immediate cellular responses to exercise: Reduced contractility and metabolism, but maintained calcium cycling and increased myofilament calcium sensitivity. Cell Biochemistry and Function, 41 (8). pp. 1147-1161. ISSN 0263-6484
See full publications listDunne, Michael
Author
Da Silva Costa, Ana and Ghouri, Iffath and Johnston, Alexander and Mcglynn, Karen and McNair, Andrew and Bowman, Peter and Malik, Natasha and Hurren, Johanne and Bingelis, Tomas and Dunne, Michael and Smith, Godfrey L. and Kemi, Ole J. (2023) Electrically stimulated in vitro heart cell mimic of acute exercise reveals novel immediate cellular responses to exercise: Reduced contractility and metabolism, but maintained calcium cycling and increased myofilament calcium sensitivity. Cell Biochemistry and Function, 41 (8). pp. 1147-1161. ISSN 0263-6484
See full publications listSmith, Godfrey L.
Author
Da Silva Costa, Ana and Ghouri, Iffath and Johnston, Alexander and Mcglynn, Karen and McNair, Andrew and Bowman, Peter and Malik, Natasha and Hurren, Johanne and Bingelis, Tomas and Dunne, Michael and Smith, Godfrey L. and Kemi, Ole J. (2023) Electrically stimulated in vitro heart cell mimic of acute exercise reveals novel immediate cellular responses to exercise: Reduced contractility and metabolism, but maintained calcium cycling and increased myofilament calcium sensitivity. Cell Biochemistry and Function, 41 (8). pp. 1147-1161. ISSN 0263-6484
See full publications listKemi, Ole J.
Author
Da Silva Costa, Ana and Ghouri, Iffath and Johnston, Alexander and Mcglynn, Karen and McNair, Andrew and Bowman, Peter and Malik, Natasha and Hurren, Johanne and Bingelis, Tomas and Dunne, Michael and Smith, Godfrey L. and Kemi, Ole J. (2023) Electrically stimulated in vitro heart cell mimic of acute exercise reveals novel immediate cellular responses to exercise: Reduced contractility and metabolism, but maintained calcium cycling and increased myofilament calcium sensitivity. Cell Biochemistry and Function, 41 (8). pp. 1147-1161. ISSN 0263-6484
See full publications listAvailable under License Creative Commons Attribution.
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