Stubbs, Harrison and Cannon, John and Knightbridge, Emily and Durrington, Charlotte and Roddis, Chloe and Gin-Sing, Wendy and Massey, Fiona and Knight, Daniel S. and Virsinskaite, Ruta and Lordan, James L. and Sear, Eleanoe and Apple-Pinguel, Joy and Morris, Eleanor and Johnson, Martin K. and Wort, Stephen J. (2024) Sendaway capillary NT-proBNP in pulmonary hypertension. BMJ Open Respiratory Research, 11 (1): e002124. ISSN 2052-4439

Abstract

Background: N-terminal pro-B-type natriuretic peptide (NT-proBNP) is a biomarker of cardiac ventricular wall stress that is incorporated into pulmonary hypertension (PH) risk stratification models. Sendaway sampling may enable patients to perform NT-proBNP tests remotely. This UK-wide study aimed to assess the agreement of sendaway NT-proBNP with standard venous NT-proBNP and to assess the effect of delayed processing.

Methods: Reference venous NT-proBNP was collected from PH patients. Samples for capillary and venous sendaway tests were collected contemporaneously, mailed to a reference laboratory and processed at 3 and 7 days using a Roche Cobas e411 device. Differences in paired measurements were analysed with Passing-Bablok regression, percentage difference plots and the % difference in risk strata.

Results: 113 patients were included in the study. 13% of day 3 capillary samples were insufficient. Day 3 capillary samples were not equivalent to reference samples (Passing Bablok analysis slope of 0.91 (95% CI 0.88 to 0.93) and intercept of 6.0 (95% CI 0.2 to 15.9)). The relative median difference was −7% and there were acceptable limits of agreement. Day 3 capillary NT-proBNP accurately risk stratified patients in 93.5% of cases. By comparison, day 3 venous results accurately risk stratified patients in 90.1% of cases and were equivalent by Passing-Bablok regression. Delayed sampling of sendaway tests led to an unacceptable level of agreement and systematically underestimated NT-proBNP.

Conclusions: Sendaway NT-proBNP sampling may provide an objective measure of right ventricular strain for virtual PH clinics. Results must be interpreted with caution in cases of delayed sampling.

People
Stubbs, Harrison
Author

Stubbs, Harrison and Cannon, John and Knightbridge, Emily and Durrington, Charlotte and Roddis, Chloe and Gin-Sing, Wendy and Massey, Fiona and Knight, Daniel S. and Virsinskaite, Ruta and Lordan, James L. and Sear, Eleanoe and Apple-Pinguel, Joy and Morris, Eleanor and Johnson, Martin K. and Wort, Stephen J. (2024) Sendaway capillary NT-proBNP in pulmonary hypertension. BMJ Open Respiratory Research, 11 (1): e002124. ISSN 2052-4439

Tárnoki, Ádám Domonkos and Tárnoki, Dávid László and Dąbrowska, Marta and Knetki-Wróblewska, Magdalena and Frille, Armin and Stubbs, Harrison and Blyth, Kevinn G. and Juul, Amanda Dandanell (2024) New developments in the imaging of lung cancer. Breathe, 20 (1): 230176. ISSN 1810-6838

See full publications list
Cannon, John
Author

Stubbs, Harrison and Cannon, John and Knightbridge, Emily and Durrington, Charlotte and Roddis, Chloe and Gin-Sing, Wendy and Massey, Fiona and Knight, Daniel S. and Virsinskaite, Ruta and Lordan, James L. and Sear, Eleanoe and Apple-Pinguel, Joy and Morris, Eleanor and Johnson, Martin K. and Wort, Stephen J. (2024) Sendaway capillary NT-proBNP in pulmonary hypertension. BMJ Open Respiratory Research, 11 (1): e002124. ISSN 2052-4439

Varian, Frances and Dick, Jennifer and Battersby, Christian and Roman, Stefan and Ablott, Jenna and Watson, Lisa and Binmahfooz, Sarah and Zafar, Hamza and Colgan, Gerry and Cannon, John and Suntharalingam, Jay and Lordan, Jim and Howard, Luke and McCabe, Colm and Wort, John and Price, Laura and Church, Colin and Hamilton, Neil and Armstrong, Iain and Hameed, Abdul and Hurdman, Judith and Elliot, Charlie and Condliffe, Robin and Wilkins, Martin and Webb, Alastair and Adlam, David and Benza, Ray L. and Rahimi, Kazem and Shojaei‐Shahrokhabadi, Mohadeseh and Lin, Nan X. and Wason, James M. S. and McIntosh, Alasdair and McConnachie, Alex and Middleton, Jennifer T. and Thompson, Roger and Kiely, David G. and Toshner, Mark and Rothman, Alexander (2024) Pulmonary hypertension: intensification and personalization of combination Rx (PHoenix): a phase IV randomized trial for the evaluation of dose‐response and clinical efficacy of riociguat and selexipag using implanted technologies. Pulmonary Circulation, 14 (1): e12337. ISSN 2045-8932

See full publications list
Knightbridge, Emily
Author

Stubbs, Harrison and Cannon, John and Knightbridge, Emily and Durrington, Charlotte and Roddis, Chloe and Gin-Sing, Wendy and Massey, Fiona and Knight, Daniel S. and Virsinskaite, Ruta and Lordan, James L. and Sear, Eleanoe and Apple-Pinguel, Joy and Morris, Eleanor and Johnson, Martin K. and Wort, Stephen J. (2024) Sendaway capillary NT-proBNP in pulmonary hypertension. BMJ Open Respiratory Research, 11 (1): e002124. ISSN 2052-4439

See full publications list
Durrington, Charlotte
Author

Stubbs, Harrison and Cannon, John and Knightbridge, Emily and Durrington, Charlotte and Roddis, Chloe and Gin-Sing, Wendy and Massey, Fiona and Knight, Daniel S. and Virsinskaite, Ruta and Lordan, James L. and Sear, Eleanoe and Apple-Pinguel, Joy and Morris, Eleanor and Johnson, Martin K. and Wort, Stephen J. (2024) Sendaway capillary NT-proBNP in pulmonary hypertension. BMJ Open Respiratory Research, 11 (1): e002124. ISSN 2052-4439

See full publications list
Roddis, Chloe
Author

Stubbs, Harrison and Cannon, John and Knightbridge, Emily and Durrington, Charlotte and Roddis, Chloe and Gin-Sing, Wendy and Massey, Fiona and Knight, Daniel S. and Virsinskaite, Ruta and Lordan, James L. and Sear, Eleanoe and Apple-Pinguel, Joy and Morris, Eleanor and Johnson, Martin K. and Wort, Stephen J. (2024) Sendaway capillary NT-proBNP in pulmonary hypertension. BMJ Open Respiratory Research, 11 (1): e002124. ISSN 2052-4439

See full publications list
Gin-Sing, Wendy
Author

Stubbs, Harrison and Cannon, John and Knightbridge, Emily and Durrington, Charlotte and Roddis, Chloe and Gin-Sing, Wendy and Massey, Fiona and Knight, Daniel S. and Virsinskaite, Ruta and Lordan, James L. and Sear, Eleanoe and Apple-Pinguel, Joy and Morris, Eleanor and Johnson, Martin K. and Wort, Stephen J. (2024) Sendaway capillary NT-proBNP in pulmonary hypertension. BMJ Open Respiratory Research, 11 (1): e002124. ISSN 2052-4439

See full publications list
Massey, Fiona
Author

Stubbs, Harrison and Cannon, John and Knightbridge, Emily and Durrington, Charlotte and Roddis, Chloe and Gin-Sing, Wendy and Massey, Fiona and Knight, Daniel S. and Virsinskaite, Ruta and Lordan, James L. and Sear, Eleanoe and Apple-Pinguel, Joy and Morris, Eleanor and Johnson, Martin K. and Wort, Stephen J. (2024) Sendaway capillary NT-proBNP in pulmonary hypertension. BMJ Open Respiratory Research, 11 (1): e002124. ISSN 2052-4439

See full publications list
Knight, Daniel S.
Author

Stubbs, Harrison and Cannon, John and Knightbridge, Emily and Durrington, Charlotte and Roddis, Chloe and Gin-Sing, Wendy and Massey, Fiona and Knight, Daniel S. and Virsinskaite, Ruta and Lordan, James L. and Sear, Eleanoe and Apple-Pinguel, Joy and Morris, Eleanor and Johnson, Martin K. and Wort, Stephen J. (2024) Sendaway capillary NT-proBNP in pulmonary hypertension. BMJ Open Respiratory Research, 11 (1): e002124. ISSN 2052-4439

See full publications list
Virsinskaite, Ruta
Author

Stubbs, Harrison and Cannon, John and Knightbridge, Emily and Durrington, Charlotte and Roddis, Chloe and Gin-Sing, Wendy and Massey, Fiona and Knight, Daniel S. and Virsinskaite, Ruta and Lordan, James L. and Sear, Eleanoe and Apple-Pinguel, Joy and Morris, Eleanor and Johnson, Martin K. and Wort, Stephen J. (2024) Sendaway capillary NT-proBNP in pulmonary hypertension. BMJ Open Respiratory Research, 11 (1): e002124. ISSN 2052-4439

See full publications list
Lordan, James L.
Author

Stubbs, Harrison and Cannon, John and Knightbridge, Emily and Durrington, Charlotte and Roddis, Chloe and Gin-Sing, Wendy and Massey, Fiona and Knight, Daniel S. and Virsinskaite, Ruta and Lordan, James L. and Sear, Eleanoe and Apple-Pinguel, Joy and Morris, Eleanor and Johnson, Martin K. and Wort, Stephen J. (2024) Sendaway capillary NT-proBNP in pulmonary hypertension. BMJ Open Respiratory Research, 11 (1): e002124. ISSN 2052-4439

See full publications list
Sear, Eleanoe
Author

Stubbs, Harrison and Cannon, John and Knightbridge, Emily and Durrington, Charlotte and Roddis, Chloe and Gin-Sing, Wendy and Massey, Fiona and Knight, Daniel S. and Virsinskaite, Ruta and Lordan, James L. and Sear, Eleanoe and Apple-Pinguel, Joy and Morris, Eleanor and Johnson, Martin K. and Wort, Stephen J. (2024) Sendaway capillary NT-proBNP in pulmonary hypertension. BMJ Open Respiratory Research, 11 (1): e002124. ISSN 2052-4439

See full publications list
Apple-Pinguel, Joy
Author

Stubbs, Harrison and Cannon, John and Knightbridge, Emily and Durrington, Charlotte and Roddis, Chloe and Gin-Sing, Wendy and Massey, Fiona and Knight, Daniel S. and Virsinskaite, Ruta and Lordan, James L. and Sear, Eleanoe and Apple-Pinguel, Joy and Morris, Eleanor and Johnson, Martin K. and Wort, Stephen J. (2024) Sendaway capillary NT-proBNP in pulmonary hypertension. BMJ Open Respiratory Research, 11 (1): e002124. ISSN 2052-4439

See full publications list
Morris, Eleanor
Author

Stubbs, Harrison and Cannon, John and Knightbridge, Emily and Durrington, Charlotte and Roddis, Chloe and Gin-Sing, Wendy and Massey, Fiona and Knight, Daniel S. and Virsinskaite, Ruta and Lordan, James L. and Sear, Eleanoe and Apple-Pinguel, Joy and Morris, Eleanor and Johnson, Martin K. and Wort, Stephen J. (2024) Sendaway capillary NT-proBNP in pulmonary hypertension. BMJ Open Respiratory Research, 11 (1): e002124. ISSN 2052-4439

See full publications list
Johnson, Martin K.
Author

Stubbs, Harrison and Cannon, John and Knightbridge, Emily and Durrington, Charlotte and Roddis, Chloe and Gin-Sing, Wendy and Massey, Fiona and Knight, Daniel S. and Virsinskaite, Ruta and Lordan, James L. and Sear, Eleanoe and Apple-Pinguel, Joy and Morris, Eleanor and Johnson, Martin K. and Wort, Stephen J. (2024) Sendaway capillary NT-proBNP in pulmonary hypertension. BMJ Open Respiratory Research, 11 (1): e002124. ISSN 2052-4439

See full publications list
Wort, Stephen J.
Author

Stubbs, Harrison and Cannon, John and Knightbridge, Emily and Durrington, Charlotte and Roddis, Chloe and Gin-Sing, Wendy and Massey, Fiona and Knight, Daniel S. and Virsinskaite, Ruta and Lordan, James L. and Sear, Eleanoe and Apple-Pinguel, Joy and Morris, Eleanor and Johnson, Martin K. and Wort, Stephen J. (2024) Sendaway capillary NT-proBNP in pulmonary hypertension. BMJ Open Respiratory Research, 11 (1): e002124. ISSN 2052-4439

See full publications list
Texts
322:333
lightbox image
323448.pdf - Published Version
Available under License Creative Commons Attribution Non-commercial.

Download (1MB) | Preview
Information
Title:
Sendaway capillary NT-proBNP in pulmonary hypertension
Creators:
Stubbs, Harrison and Cannon, John and Knightbridge, Emily and Durrington, Charlotte and Roddis, Chloe and Gin-Sing, Wendy and Massey, Fiona and Knight, Daniel S. and Virsinskaite, Ruta and Lordan, James L. and Sear, Eleanoe and Apple-Pinguel, Joy and Morris, Eleanor and Johnson, Martin K. and Wort, Stephen J.
Identification Number:
10.1136/bmjresp-2023-002124
Date:
22 March 2024
Divisions:
College of Medical Veterinary and Life Sciences > School of Cancer Sciences
Publisher:
BMJ Publishing Group
Additional Information:
Funding: This work was supported by funding from Janssen- Cilag. Dr DS Knight is supported by a British Heart Foundation (BHF) Clinical Research Leave Fellowship (FS/CRLF/20/23004).
Library
URI:
https://pub.demo35.eprints-hosting.org/id/eprint/322
Item Type:
Article
Depositing User:
Unnamed user with email ejo1f20@soton.ac.uk
SWORD Depositor:
Users 37347 not found.
Date Deposited:
11 Jun 2025 16:37
Revision:
13
Last Modified:
12 Jun 2025 09:56
View Item