Kapitany, Valentin and Fatima, Areeba and Zickus, Vytautas and Whitelaw, Jamie and McGhee, Ewan and Insall, Robert and Machesky, Laura and Faccio, Daniele (2024) Single-sample image-fusion upsampling of fluorescence lifetime images. Science Advances, 10 (21): eadn0139. ISSN 2375-2548
AI Summary:
Single-sample image-fusion upsampling is a data-fusion approach to computational FLIM super-resolution. It combines measurements from a low-resolution time-resolved detector and a high-resolution camera.AI Topics:
Fluorescence lifetime imaging microscopy (FLIM) provides detailed information about molecular interactions and biological processes. A major bottleneck for FLIM is image resolution at high acquisition speeds due to the engineering and signal-processing limitations of time-resolved imaging technology. Here, we present single-sample image-fusion upsampling, a data-fusion approach to computational FLIM super-resolution that combines measurements from a low-resolution time-resolved detector (that measures photon arrival time) and a high-resolution camera (that measures intensity only). To solve this otherwise ill-posed inverse retrieval problem, we introduce statistically informed priors that encode local and global correlations between the two “single-sample” measurements. This bypasses the risk of out-of-distribution hallucination as in traditional data-driven approaches and delivers enhanced images compared, for example, to standard bilinear interpolation. The general approach laid out by single-sample image-fusion upsampling can be applied to other image super-resolution problems where two different datasets are available.
Kapitany, Valentin
Author
Kapitany, Valentin and Fatima, Areeba and Zickus, Vytautas and Whitelaw, Jamie and McGhee, Ewan and Insall, Robert and Machesky, Laura and Faccio, Daniele (2024) Single-sample image-fusion upsampling of fluorescence lifetime images. Science Advances, 10 (21): eadn0139. ISSN 2375-2548
See full publications listFatima, Areeba
Author
Kapitany, Valentin and Fatima, Areeba and Zickus, Vytautas and Whitelaw, Jamie and McGhee, Ewan and Insall, Robert and Machesky, Laura and Faccio, Daniele (2024) Single-sample image-fusion upsampling of fluorescence lifetime images. Science Advances, 10 (21): eadn0139. ISSN 2375-2548
See full publications listZickus, Vytautas
Author
Kapitany, Valentin and Fatima, Areeba and Zickus, Vytautas and Whitelaw, Jamie and McGhee, Ewan and Insall, Robert and Machesky, Laura and Faccio, Daniele (2024) Single-sample image-fusion upsampling of fluorescence lifetime images. Science Advances, 10 (21): eadn0139. ISSN 2375-2548
See full publications listWhitelaw, Jamie
Author
Nikolaou, Savvas and Juin, Amelie and Whitelaw, Jamie A. and Paul, Nikki R. and Fort, Loic and Nixon, Colin and Spence, Heather J. and Bryson, Sheila and Machesky, Laura M. (2024) CYRI-B-mediated macropinocytosis drives metastasis via lysophosphatidic acid receptor uptake. eLife, 13: e83712. ISSN 2050-084X
Kapitany, Valentin and Fatima, Areeba and Zickus, Vytautas and Whitelaw, Jamie and McGhee, Ewan and Insall, Robert and Machesky, Laura and Faccio, Daniele (2024) Single-sample image-fusion upsampling of fluorescence lifetime images. Science Advances, 10 (21): eadn0139. ISSN 2375-2548
See full publications listMcGhee, Ewan
Author
Kapitany, Valentin and Fatima, Areeba and Zickus, Vytautas and Whitelaw, Jamie and McGhee, Ewan and Insall, Robert and Machesky, Laura and Faccio, Daniele (2024) Single-sample image-fusion upsampling of fluorescence lifetime images. Science Advances, 10 (21): eadn0139. ISSN 2375-2548
See full publications listInsall, Robert
Author
Farndale, Lucas and Insall, Robert and Yuan, Ke (2025) TriDeNT: Triple deep network training for privileged knowledge distillation in histopathology. Medical Image Analysis, 102: 103479. ISSN 1361-8415
Mandrou, Elena and Thomason, Peter A. and Paschke, Peggy I. and Paul, Nikki R. and Tweedy, Luke and Insall, Robert (2024) A reliable system for quantitative g-protein activation imaging in cancer cells. Cells, 13 (13): 1114. ISSN 2073-4409
Kapitany, Valentin and Fatima, Areeba and Zickus, Vytautas and Whitelaw, Jamie and McGhee, Ewan and Insall, Robert and Machesky, Laura and Faccio, Daniele (2024) Single-sample image-fusion upsampling of fluorescence lifetime images. Science Advances, 10 (21): eadn0139. ISSN 2375-2548
See full publications listMachesky, Laura
Author
Nikolaou, Savvas and Juin, Amelie and Whitelaw, Jamie A. and Paul, Nikki R. and Fort, Loic and Nixon, Colin and Spence, Heather J. and Bryson, Sheila and Machesky, Laura M. (2024) CYRI-B-mediated macropinocytosis drives metastasis via lysophosphatidic acid receptor uptake. eLife, 13: e83712. ISSN 2050-084X
Kapitany, Valentin and Fatima, Areeba and Zickus, Vytautas and Whitelaw, Jamie and McGhee, Ewan and Insall, Robert and Machesky, Laura and Faccio, Daniele (2024) Single-sample image-fusion upsampling of fluorescence lifetime images. Science Advances, 10 (21): eadn0139. ISSN 2375-2548
Juin, Amelie and Spence, Heather J. and Machesky, Laura M. (2024) Dichotomous role of the serine/threonine kinase MAP4K4 in pancreatic ductal adenocarcinoma onset and metastasis through control of AKT and ERK pathways. Journal of Pathology, 262 (4). pp. 454-466. ISSN 0022-3417
See full publications listFaccio, Daniele
Author
Kapitany, Valentin and Fatima, Areeba and Zickus, Vytautas and Whitelaw, Jamie and McGhee, Ewan and Insall, Robert and Machesky, Laura and Faccio, Daniele (2024) Single-sample image-fusion upsampling of fluorescence lifetime images. Science Advances, 10 (21): eadn0139. ISSN 2375-2548
See full publications listAvailable under License Creative Commons Attribution.
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