Simpson, Annabel and Johnston, William and Carda-Diéguez, Miguel and Mira, Alex and Easton, Chris and Henriquez, Fiona L. and Culshaw, Shauna and Rosier, Bob T. and Burleigh, Mia (2024) Periodontal treatment causes a longitudinal increase in nitrite-producing bacteria. Molecular Oral Microbiology, 39 (6). pp. 491-506. ISSN 2041-1006
AI Summary:
Periodontal treatment induced an immediate increase in the relative abundance of health-associated NO2-producing bacteria. This increase persisted throughout periodontal healing.AI Topics:
Background
The oral microbiome-dependent nitrate (NO3−)–nitrite (NO2−)–nitric oxide (NO) pathway may help regulate blood pressure. NO2−-producing bacteria in subgingival plaque are reduced in relative abundance in patients with untreated periodontitis compared with periodontally healthy patients. In periodontitis patients, the NO2−-producing bacteria increase several months after periodontal treatment. The early effects of periodontal treatment on NO2−-producing bacteria and the NO3−–NO2−–NO pathway remain unknown. The aim of this study was to determine how periodontal treatment affects the oral NO2−-producing microbiome and salivary NO3− and NO2− levels over time.
Methods
The subgingival microbiota of 38 periodontitis patients was analysed before (baseline [BL]) and 1, 7 and 90 days after periodontal treatment. Changes in NO2−-producing bacteria and periodontitis-associated bacteria were determined by 16s rRNA Illumina sequencing. Saliva samples were collected at all-time points to determine NO3− and NO2− levels using gas-phase chemiluminescence.
Results
A significant increase was observed in the relative abundance of NO2−-producing species between BL and all subsequent timepoints (all p < 0.001). Periodontitis-associated species decreased at all timepoints, relative to BL (all p < 0.02). NO2−-producing species negatively correlated with periodontitis-associated species at all timepoints, with this relationship strongest 90 days post-treatment (ρ = −0.792, p < 0.001). Despite these findings, no significant changes were found in salivary NO3− and NO2− over time (all p > 0.05).
Conclusions
Periodontal treatment induced an immediate increase in the relative abundance of health-associated NO2−-producing bacteria. This increase persisted throughout periodontal healing. Future studies should test the effect of periodontal treatment combined with NO3− intake on periodontal and cardiovascular health.
Simpson, Annabel
Author
Simpson, Annabel and Johnston, William and Carda-Diéguez, Miguel and Mira, Alex and Easton, Chris and Henriquez, Fiona L. and Culshaw, Shauna and Rosier, Bob T. and Burleigh, Mia (2024) Periodontal treatment causes a longitudinal increase in nitrite-producing bacteria. Molecular Oral Microbiology, 39 (6). pp. 491-506. ISSN 2041-1006
Rosier, Bob T. and Johnston, William and Carda-Diéguez, Miguel and Simpson, Annabel and Cabello-Yeves, Elena and Piela, Krystyna and Reilly, Robert and Artacho, Alejandro and Easton, Chris and Burleigh, Mia and Culshaw, Shauna and Mira, Alex (2024) Publisher Correction: Nitrate reduction capacity of the oral microbiota is impaired in periodontitis: potential implications for systemic nitric oxide availability. International Journal of Oral Science, 16: 8. ISSN 1674-2818
See full publications listJohnston, William
Author
Ware, Alicia and Johnston, William and Delaney, Christopher and Butcher, Mark C. and Ramage, Gordon and Price, Lesley and Butcher, John and Kean, Ryan (2025) Dry surface biofilm formation by Candida auris facilitates persistence and tolerance to sodium hypochlorite. APMIS: Journal of Pathology, Microbiology and Immunology, 133 (4): e70022. ISSN 0903-4641
Simpson, Annabel and Johnston, William and Carda-Diéguez, Miguel and Mira, Alex and Easton, Chris and Henriquez, Fiona L. and Culshaw, Shauna and Rosier, Bob T. and Burleigh, Mia (2024) Periodontal treatment causes a longitudinal increase in nitrite-producing bacteria. Molecular Oral Microbiology, 39 (6). pp. 491-506. ISSN 2041-1006
Johnston, William and Ware, Alicia and Kuiters, Willemijn Frederique and Delaney, Christopher and Brown, Jason Lee and Hagen, Suzanne and Corcoran, David and Cummings, Matthew and Ramage, Gordon and Kean, Ryan (2023) In vitro bacterial vaginosis biofilm community manipulation using endolysin therapy. Biofilm, 5: 100101. ISSN 2590-2075
See full publications listCarda-Diéguez, Miguel
Author
Simpson, Annabel and Johnston, William and Carda-Diéguez, Miguel and Mira, Alex and Easton, Chris and Henriquez, Fiona L. and Culshaw, Shauna and Rosier, Bob T. and Burleigh, Mia (2024) Periodontal treatment causes a longitudinal increase in nitrite-producing bacteria. Molecular Oral Microbiology, 39 (6). pp. 491-506. ISSN 2041-1006
Rosier, Bob T. and Johnston, William and Carda-Diéguez, Miguel and Simpson, Annabel and Cabello-Yeves, Elena and Piela, Krystyna and Reilly, Robert and Artacho, Alejandro and Easton, Chris and Burleigh, Mia and Culshaw, Shauna and Mira, Alex (2024) Publisher Correction: Nitrate reduction capacity of the oral microbiota is impaired in periodontitis: potential implications for systemic nitric oxide availability. International Journal of Oral Science, 16: 8. ISSN 1674-2818
See full publications listMira, Alex
Author
Simpson, Annabel and Johnston, William and Carda-Diéguez, Miguel and Mira, Alex and Easton, Chris and Henriquez, Fiona L. and Culshaw, Shauna and Rosier, Bob T. and Burleigh, Mia (2024) Periodontal treatment causes a longitudinal increase in nitrite-producing bacteria. Molecular Oral Microbiology, 39 (6). pp. 491-506. ISSN 2041-1006
Rosier, Bob T. and Johnston, William and Carda-Diéguez, Miguel and Simpson, Annabel and Cabello-Yeves, Elena and Piela, Krystyna and Reilly, Robert and Artacho, Alejandro and Easton, Chris and Burleigh, Mia and Culshaw, Shauna and Mira, Alex (2024) Publisher Correction: Nitrate reduction capacity of the oral microbiota is impaired in periodontitis: potential implications for systemic nitric oxide availability. International Journal of Oral Science, 16: 8. ISSN 1674-2818
See full publications listEaston, Chris
Author
Simpson, Annabel and Johnston, William and Carda-Diéguez, Miguel and Mira, Alex and Easton, Chris and Henriquez, Fiona L. and Culshaw, Shauna and Rosier, Bob T. and Burleigh, Mia (2024) Periodontal treatment causes a longitudinal increase in nitrite-producing bacteria. Molecular Oral Microbiology, 39 (6). pp. 491-506. ISSN 2041-1006
Rosier, Bob T. and Johnston, William and Carda-Diéguez, Miguel and Simpson, Annabel and Cabello-Yeves, Elena and Piela, Krystyna and Reilly, Robert and Artacho, Alejandro and Easton, Chris and Burleigh, Mia and Culshaw, Shauna and Mira, Alex (2024) Publisher Correction: Nitrate reduction capacity of the oral microbiota is impaired in periodontitis: potential implications for systemic nitric oxide availability. International Journal of Oral Science, 16: 8. ISSN 1674-2818
See full publications listHenriquez, Fiona L.
Author
Simpson, Annabel and Johnston, William and Carda-Diéguez, Miguel and Mira, Alex and Easton, Chris and Henriquez, Fiona L. and Culshaw, Shauna and Rosier, Bob T. and Burleigh, Mia (2024) Periodontal treatment causes a longitudinal increase in nitrite-producing bacteria. Molecular Oral Microbiology, 39 (6). pp. 491-506. ISSN 2041-1006
See full publications listCulshaw, Shauna
Author
Warner, Frances and Sherriff, Andrea and Culshaw, Shauna and Holliday, Richard and Ryan, Vicky and Conway, David I. (2024) Trends and socioeconomic inequalities in the dental attendance of adult smokers in Scotland from 2009 to 2019, a repeated cross-sectional study. BMC Public Health, 24 (1): 2156. ISSN 1471-2458
Simpson, Annabel and Johnston, William and Carda-Diéguez, Miguel and Mira, Alex and Easton, Chris and Henriquez, Fiona L. and Culshaw, Shauna and Rosier, Bob T. and Burleigh, Mia (2024) Periodontal treatment causes a longitudinal increase in nitrite-producing bacteria. Molecular Oral Microbiology, 39 (6). pp. 491-506. ISSN 2041-1006
West, Nicola and Chapple, Ian and Culshaw, Shauna and Donos, Nikos and Needleman, Ian and Suvan, Jeanie and Nibali, Luigi and Patel, Amit and Preshaw, Philip M. and Kebschull, Moritz and EFP workshop participants and methodological consultant, . (2024) BSP implementation of prevention and treatment of peri-implant diseases - the EFP S3 level clinical practice guideline. Journal of Dentistry, 149: 104980. ISSN 0300-5712
See full publications listRosier, Bob T.
Author
Simpson, Annabel and Johnston, William and Carda-Diéguez, Miguel and Mira, Alex and Easton, Chris and Henriquez, Fiona L. and Culshaw, Shauna and Rosier, Bob T. and Burleigh, Mia (2024) Periodontal treatment causes a longitudinal increase in nitrite-producing bacteria. Molecular Oral Microbiology, 39 (6). pp. 491-506. ISSN 2041-1006
Rosier, Bob T. and Johnston, William and Carda-Diéguez, Miguel and Simpson, Annabel and Cabello-Yeves, Elena and Piela, Krystyna and Reilly, Robert and Artacho, Alejandro and Easton, Chris and Burleigh, Mia and Culshaw, Shauna and Mira, Alex (2024) Publisher Correction: Nitrate reduction capacity of the oral microbiota is impaired in periodontitis: potential implications for systemic nitric oxide availability. International Journal of Oral Science, 16: 8. ISSN 1674-2818
See full publications listBurleigh, Mia
Author
Simpson, Annabel and Johnston, William and Carda-Diéguez, Miguel and Mira, Alex and Easton, Chris and Henriquez, Fiona L. and Culshaw, Shauna and Rosier, Bob T. and Burleigh, Mia (2024) Periodontal treatment causes a longitudinal increase in nitrite-producing bacteria. Molecular Oral Microbiology, 39 (6). pp. 491-506. ISSN 2041-1006
Rosier, Bob T. and Johnston, William and Carda-Diéguez, Miguel and Simpson, Annabel and Cabello-Yeves, Elena and Piela, Krystyna and Reilly, Robert and Artacho, Alejandro and Easton, Chris and Burleigh, Mia and Culshaw, Shauna and Mira, Alex (2024) Publisher Correction: Nitrate reduction capacity of the oral microbiota is impaired in periodontitis: potential implications for systemic nitric oxide availability. International Journal of Oral Science, 16: 8. ISSN 1674-2818
See full publications listAvailable under License Creative Commons Attribution.
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